- 牛昱宇（Yuyu Niu）“国家高层次人才”特聘教授项目入选者、科技部重点研发计划项目首席科学家昆明理工大学生命科学与技术学院 院长省部共建非人灵长类生物医学国家重点实验室 副主任Dean & Professor of Faculty of Life Science and Technology, KUSTAssociate Director and Professor of State Key Laboratory of Primate Biomedical Research
一、教育经历 Education
1995年 - 1999年，云南师范大学，生命科学系，本科
2001年 - 2007年，中国科学院昆明动物研究所，博士
2006年 - 2008年，德国柏林野生与笼养动物研究所，访问学者
1.1995-1999, Yunnan Normal University, Bachelor of Biology.
2.2001-2007, Kunming Institute Of Zoology, Chinese Academy of Sciences & Zoological Research, Ph.D.
3.2006-2008, Leibniz Institute for Zoo and Wildlife Research, Visiting Scholar.
二、工作经历 Work Experience
2019年11月 -，昆明理工大学生命科学与技术学院，教授，院长
2014年12月 -，昆明理工大学灵长类转化医学研究院，教授
2011年07月 -，省部共建非人灵长类生物医学国家重点实验室，副主任
2010年09月 - 2014年05月，中科院昆明动物研究所，副研究员
2007年04月 - 2010年09月，中科院昆明动物研究所，助理研究员
1.2019-present, Dean and Professor, Faculty of Life Science and Technology, Kunming University of Science and Technology.
2.2014-present, Professor, Institute of Primate Translational Medicine, Kunming University of Science and Technology.
2.2011-present, Associate Director, State Key Laboratory of Primate Biomedical Research.
3.2010-2014, Associate Professor, Kunming Institute Of Zoology,Chinese Academy of Sciences & Zoological Research.
4.2007-2010, Assistant professor, Kunming Institute Of Zoology,Chinese Academy of Sciences & Zoological Research.
三、主要研究方向 Main Research Directions
灵长类基因编辑; 灵长类胚胎发育与疾病
Gene editing, Primate embryonic development
四、科研领域描述 Description of Scientific Research
早期胚胎发育是哺乳动物生命发生的重要阶段，这一时期的关键生物学事件，如合子基因启动，表观遗传维持与重建，胚层分化和器官形成等等，是发育生物学的重要基础理论，又和人类健康和疾病密切相关。然而，我们对其中的诸多科学问题却知之甚少。本实验室结合灵长类早期胚胎（体外受精，单性生殖和体细胞核移植）培养，基因编辑，干细胞与示踪，单细胞组学和灵长类疾病动物模型等技术体系，系统研究灵长类细胞多能性的维持与重建，表观遗传调控与胚层分化，退行疾病的早期诊断和干预等重要科学问题，以期揭示人类生命发生和疾病形成的奥秘。
Research in Yuyu Niu's laboratory is focused on early embryo development and reprogramming, particularly preimplantation embryo development which play an important role in cell differentiation and organ initiation, and therefore have a significant on human disease. Over the last decade, Dr. Niu has made significant contributions to non-human primate genetic engineering and early embryo development that include: 1) the first transgenic rhesus monkey in China (PNAS 2010); 2) the first monkey model with genome engineered by precision gene-targeting methods (Cell 2014; Cell 2017); 3) the continuous development of monkey embryos in vitro beyond early gastrulation and to 20 days post fertilization (Science 2019). Built on the strength in non-human primate research, the Yuyu Niu's laboratory has expanded its capability to use a variety of state-of-the-art techniques, including single cell transcriptomics, cell lineage tracing, stem cells, somatic cell nuclear transfer and CRISPR/Cas9-based genomic editing to understand the molecular events at the beginning of primate life and cell reprogramming. In addition, the lab has studied on mechanism and biomarker of neurodegeneration diseases, such as Parkinson’s disease and Amyotrophic lateral sclerosis (ALS).
五、承担科研项目情况 Research Projects
- 国家重点研发计划“发育编程及其代谢调节”项目，灵长类原肠发育与器官形成的命运决定调控机制，2021-2026，3100万元
- 国家自然科学基金联合基金项目，利用单碱基编辑技术构建早衰症猴模型及其机理研究，2022-2025，233万元
- 国家自然科学基金面上项目，肌萎缩侧索硬化症灵长类动物转基因模型的建立及早期发病机制研究，2021-2024，58万元
六、获奖情况
- 2008年获云南省自然科学类二等奖
- 2012年获中国科学院卢嘉锡青年人才奖
- 2014年获云南省青年科技奖
- 2017年获云南省自然科学奖一等奖
- 2021年获云南省自然科学奖一等奖
七、代表性论文（近5年） Representative papers
1. Yu Kang#, Shaoxing Dai#, Yuqiang Zeng#, Fang Wang#, Pengpeng Yang, Zhaohui Yang, Youwei Pu1,3, Zifan Li, Xinglong Chen1, Baohong Tian, Wei Si, Weizhi Ji*, Yuyu Niu*. Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis. Science Advances. 2022.7 8(29)：1-11 [IF: 14.957]
2. Zhu X, G. Y., Chu C, Liu D, Duan K, Yin Y, Si C, Kang Y, Yao J, Du X, Li J, Zhao S, Ai Z, Zhu Q, Ji W*, Niu Y*, Li T*. BRN2 as a key gene drives the early primate telencephalon development Science Advances, doi:10.1126/sciadv.abl7263 (2022). (* co-corresponding author)
3. Zhong T, W. J., Wu K, Chen L, Zhao F, Pei Y, Wang Y, Zhang H, Wu Z, Huang Y, Li T, Wang L, Chen Y, Ji W, Zhang Y, Li G*, Niu Y*. Longitudinal brain atlases of early developing cynomolgus macaques from birth to 48 months of age. Neuroimage 247, 118799, doi:10.1016/j.neuroimage.2021.118799 (2022). (* co-corresponding author)
4. Yang R, G. A., Kang Y, Si C, Chu C, Zheng Y, Chen Z, Gruber PJ, Xiao Y, Zhou C, Witman N, Eroglu E, Leung CY, Chen Y, Fu J, Ji W*, Lanner F*, Niu Y*, Chien KR*. Amnion signals are essential for mesoderm formation in primates. Nature communications 12, 5126 (2021). (* co-corresponding author)
5. Tan T*, W. J., Si C, Dai S, Zhang Y, Sun N, Zhang E, Shao H, Si W, Yang P, Wang H, Chen Z, Zhu R, Kang Y, Hernandez-Benitez R, Martinez Martinez L, Nuñez Delicado E, Berggren WT, Schwarz M, Ai Z, Li T, Rodriguez Esteban C, Ji W*, Niu Y*, Izpisua Belmonte JC*. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo. Cell 184, 2020-2032 e2014, doi:10.1016/j.cell.(2021). (* co-corresponding author)
6. Chu C, Z. W*. , Kang Y, Si C, Ji W*, Niu Y*, Zhang Y*. Analysis of developmental imprinting dynamics in primates using SNP-free methods to identify imprinting defects in cloned placenta. Developmental cell 56, 2826-2840. e2827 (2021). (* co-corresponding author)
7. Chen ZZ, W. J., Kang Y, Yang QY, Gu XY, Zhi DL, Yan L, Long CZ, Shen B*, Niu YY*. PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys. Zoological Research 42, 469 (2021). (* co-corresponding author)
8. Chen C, J. W., Niu Y*. Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research. Trends in Biotechnology 39(12):1332-1342 (2021).
9. Wang F, Z. W., Yang Q, Kang Y, Fan Y, Wei J, Liu Z, Dai S, Li H, Li Z, Xu L, Chu C, Qu J, Si C, Ji W*, Liu GH*, Long C*, Niu Y*. Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing. Protein & cell 11, 809-824 (2020). (* co-corresponding author)
10. Li B, N. Y*., Ji W*, Dong Y*. Strategies for the CRISPR-based therapeutics. Trends in pharmacological sciences 41, 55-65 (2020).(* co-corresponding author)
11. Wang D, N. Y*., Ren L, Kang Y, Tai PWL, Si C, Mendonca CA, Ma H, Gao G, Ji W. Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus. Advanced Science 6, 1900440 (2019). (* co-first author)
12. Niu Y*, S. N., Li C, Lei Y, Huang Z, Wu J, Si C, Dai X, Liu C, Wei J, Liu L, Feng S, Kang Y, Si W, Wang H, Zhang E, Zhao L, Li Z, Luo X, Cui G, Peng G, Izpisúa Belmonte JC*, Ji W*, Tan T*. Dissecting primate early post-implantation development using long-term in vitro embryo culture. Science 366 (2019). (* co-corresponding author)
13. Kang Y, C. C., Wang F, Niu Y*. CRISPR/Cas9-mediated genome editing in nonhuman primates. Disease models & mechanisms 12, dmm039982 (2019).
