• 司维(Wei Si)

    副院长

    教授,博士生导师

    一、受教育及工作经历 Education and Working Experience

    1993年9月-1997年7月,云南大学生物系,生物学专业,获生物学学士学位
    1997年9月-2004年2月,中科院昆明动物研究所,动物学专业,生殖生物学研究方向获动物学博士学位
    2004年2月–2005年2月,中科院昆明动物研究所,实习研究员
    2005年2月–2007年11月,美国University of Missouri-Columbia,博士后
    2007年11月-2008年11月,美国 Medical College of Georgia,博士后
    2008年12月-2014年12月,中科院昆明动物研究所,副研究员
    2011年12月至今,云南中科灵长类生物医学重点实验室,研究员
    2014年12月至今,昆明理工大学灵长类转化医学研究院,教授

     

    1993-1997, Bachelor's Degree, Department of Biology, Yunnan University.

    1997-2004, PhD, Kunming Institute of Zoology, Chinese Academy of Sciences.

    2004-2005, Research assistant, Kunming Institute of Zoology, Chinese Academy of Sciences.

    2005-2007, Postdoctoral, University of Missouri-Columbia, USA.

    2007-2008, Postdoctoral, Medical College of Georgia, USA.

    2009-2014,Associate Professor, Kunming Institute of Zoology, Chinese Academy of Sciences.

    Since 2014, Professor, Institute of Primate Translational Medicine, Kunming University of Technology.

    二、主要研究方向 Research Interests

    人类疾病的灵长类动物模型建立和细胞/基因治疗的有效性和安全性评价的临床前研究,人类疾病与肠道菌群之间关系的动物实验模型,灵长类种质资源(精子、卵母细胞和胚胎)和干细胞细胞的超低温保存的冷冻损伤机制和提高保存效率的对策;灵长类发育过程中细胞分化的基因组表达和甲基化调控。

     

    Establishment of primate animal models of human diseases and pre-clinical studies on effectiveness and safety of cell and gene therapy; Relationship between diseases and intestinal microorganisms; Mechanism of freezing injury in cryopreservation of germ cells and stem cell cells; Regulation of genomic expression during primate embryonic development.

    三、承担科研项目情况 Research Projects and Grants

    1. 国家重点研发计划项目:发育代谢复杂疾病灵长类动物模型的创制与临床前研究,2018YFA0801400, 2019-2024,7050万元,首席科学家。
    2. 国家自然科学基金面上项目:猕猴肝纤维化疾病模型间充质干细胞移植治疗的细胞示踪以及移植细胞基因表达调控的临床前研究(31872973),2019-2022年,58万,主持。
    3. 国家自然科学基金地区基金项目:灵长类动物早期着床胚胎三胚层分化和器官发生基因组表达调控的研究(31660346),2017-2020年,43万,主持。
    4. 国家重点研发计划“干细胞及转化研究”试点专项:靶向基因编辑建立神经系统疾病猴模型及干细胞治疗研究(子课题2016YFA0101400),2017-2020年,320万,骨干。
    5. 云南省科技计划项目:人脐带MSC临床适用性关键技术建立及其在猕猴肝纤移植治疗中的应用(2018ZF7-05), 2018-2020, 140万,骨干。
    6. 国家重大科学研究计划重大科学问题目标导向项目 “细胞多能性和人类重大疾病的猴模型研究” 子课题:猕猴肝损伤动物模型(2012CBA01305),2012-2016年,129.5万,主持。
    1. National Key Research and Development Project (2018YFA0801400), 2019-2024,¥70,050,000, Chief scientist.
    2. National Natural Science Foundation of China (31872973), 2019-2022, ¥580,000, PI.
    3. Natural Science Foundation of China (31660346), 2017-2020, ¥430,000, PI.
    4. National Key Research and Development Program of China (2016YFA0101403), 2016-2020, ¥3,200,000, Co-PI.
    5. Yunnan Science and Technology Project (2018ZF7-05), 2018-2020, ¥1,400,000, Co-PI.
    6. The National Basic Research Program of China (2012CBA01305), 2012-2016, ¥1,295,000, PI.
     

    四、获奖情况 Awards

     

    1. 司维,季维智,李亚辉,郑萍,牛昱宇,和协超,纪少珲。生物医学实验动物精子超低温冷冻保存,云南省自然科学奖,三等奖,2012。
    2. 王宏,季维智,严晔,牛昱宇,司维,纪少珲,马原野。标准化灵长类实验动物国际认证建设与应用,云南省科学技术进步奖,三等奖,2015。
    3. 季维智,牛昱宇,陈永昌,司维,王宏,康宇,司晨洋。非人灵长类基因编辑与神经系统疾病模型研究,云南省自然科学奖,一等奖,2017。
     

    五、代表性论文和专著 Publications

    1. Xufeng Fu # , Bo Xu # , Jiang Jiang # , Xing Du , Xiaoli Yu , Yaping Yan , Shanshan Li , Briauna Marie Inglis , Huiming Ma , Hongyan Wang , Xiuying Pei , Wei Si . Effects of cryopreservation and long-term culture on biological characteristics and proteomic profiles of human umbilical cord-derived mesenchymal stem cells. Clin Proteomics. 2020;17:15. Published 2020 May 24. doi:10.1186/s12014-020-09279-6
    2. Junfeng Wang , Xufeng Fu , Yaping Yan , Shanshan Li , Yanchao Duan , Briauna Marie Inglis Wei Si , Bingrong Zheng. In vitro differentiation of rhesus macaque bone marrow- and adipose tissue-derived MSCs into hepatocyte-like cells. Exp Ther Med. 2020;20(1):251-260. doi:10.3892/etm.2020.8676
    3. Bin Jiang, Xufeng Fu, Li Yan, Shanshan Li, Dongliang Zhao, Xiaoyan Wang, Yanchao Duan, Yaping Yan, Enqin Li, Kunhua Wu, Briauna Marie Inglis, Weizhi Ji, Ren-He Xu, Wei Si. Transplantation of human ESC-derived mesenchymal stem cell spheroids ameliorates spontaneous osteoarthritis in rhesus macaques. Theranostics. 2019, 9: 6587-6600.
    4. Shengnan Wang, Yanchao Duan, Yaping Yan, Chen Adar, Ido Braslavsky, Bingbing Chen, Tianzhuang Huang, Shuai Qiu, Xi Li, Briauna Marie Inglis, Weizhi Ji, Wei Si*. Improvement of sperm cryo-survival of cynomolgus macaque (Macaca fascicularis) by commercial egg-yolk–free freezing medium with type III antifreeze protein. Animal Reproduction Science. 2019, 210:106177.
    5. Shanshan Li, Lei Ao, Yaping Yan, Jiang Jiang, Bingbing Chen, Yanchao Duan, Fei Shen, Jinbao Chen, Briauna Inglis, Renmin Ni, Weizhi Ji*, Wei Si*. Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial. Clinical Proteomics. 2019, 16:24.
    6. Hong Wang, Yaping Yan, Xiaoyan Yi, Yanchao Duan, Junfeng Wang, Shanshan Li, Lilin Luo, Tianzhuang Huang, Briauna Inglis, Xi Li, Weizhi Ji, Tao Tan*, Wei Si*. Histopathological features and composition of gut microbiota in rhesus monkey of alcoholic liver disease. Frontiers in Microbiology. 2019, 10: 165.
    7. Yao Wang, Hanben Wang, Yu Zhang, Zhenhai Du, Wei Si, Suixing Fan, Dongdong Qin, Mei Wang, Yanchao Duan, Lufan Li, Yuying Jiao, Yuanyuan Li, Qiujun Wang, Qinghua Shi, Xin Wu, Wei Xie. Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis. Molecular Cell. 2019, 73:547-561.
    8. Li Yan #, Bin Jiang #, Yuyu Niu #, Hongxuan Wang, Enqin Li, Yaping Yan, Huiyan Sun, Yanchao Duan, Shaohui Chang, Guokai Chen, Weizhi Ji*, Ren-He Xu*, Wei Si*. Intrathecal delivery of human ESC-derived mesenchymal stem cell spheres promotes recovery of a primate multiple sclerosis model. Cell Death Discov. 2018, 5:28.
    9. Yang Jiao, Hongbo Zhao, Guodong Chen, Xiongbo Sang, Luhan Yang, Zongliu Hou*, Wei Si*, Bingrong Zheng*. Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs. Stem Cells Int. 2018:5912194.
    10. Xufeng Fu, Bin Jiang, Bingrong Zheng, Yaping Yan, Junfeng Wang, Yanchao Duan, Shanshan Li, Li Yan, Hong Wang, Bingbing Chen, Xiongbo Sang, Weizhi Ji*, Ren-He Xu*, Wei Si*. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse. PeerJ. 2018, 6:e4336.
    11. Xufeng Fu, Yaping Yan, Shanshan Li, Junfeng Wang, Bin Jiang, Hong Wang, Yanchao Duan, Tao Tan, Fei Gao, Desheng Gong, Yuyu Niu, Weizhi Ji*, Wei Si* et al. Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression. Stem Cells Int. 2017:3893691.
    12. Bingbing Chen, Shanshan Li, Yaping Yan, Yanchao Duan, Shaohui Chang, Hong Wang, Weizhi Ji*, Xin Wu*, Wei Si*. Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels - CatSper and Hv1. Theriogenology. 2017, 104:37-42.
    13. Yan Yaping, Ao Lei, Wang Hong, Duan Yanchao, Chang Shaohui, Chen Bingbing, Zhi Dalong, Li Sujuan, Niu Yuyu, Ji Weizhi, Si Wei*. Optimization of cryopreservation of cyomolgus macaque (Macaca fascicularis) sperm with commercial egg yolk free SpermCryo All-round medium. J Am Assoc Lab Anim Sci. 2016, 55:744-748.
    14. Hong Wang, Tao Tan, Junfeng Wang, Yuyu Niu, Yaping Yan, Xiangyu Guo, Yu Kang, Yanchao Duan, Shaohui Chang, Jianpeng Liao, Chenyang Si, Weizhi Ji*, Wei Si*. Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis. Sci Rep. 2015;5:15019.
    15. Shihua Yang, Shuhuang Ping, Shaohui Ji, Yongqing Lu, Yuyu Niu, Hong Wang, Weizhi Ji, Wei Si*. The positive effects of seminal plasma during freezing process on cryosurvival of sperm with poor freezability in Rhesus Macaque (Macaca mulatta). J Reprod Dev. 2011, 57:737-43.
    16. Shihua Yang, Shuhuang Ping, Wei Si*, Xiechao He, Xinyi Wang, Yongqing Lu, Shaohui Ji, Yuyu Niu, Weizhi Ji. Optimize Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Sperm Cryopreservation of Rhesus Macaque (Macaca mulatta). J Vet Med Sci. 2011, 73:717-723.
    17. Wei Si, Yongqing Lu, Xiechao He, Shaohui Ji, Yuyu Niu, Tao Tan, Weizhi Ji. Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm. Theriogenology. 2010, 74:1431–1438.
    18. Wei Si, Hongsheng Men, James D Benson, John K Critser. Osmotic characteristics and fertility of murine spermatozoa collected in different solutions. Reproduction. 2009, 137:215-223.
    19. Nancy A Rodriguez, Wei Si, Adelina M Emmi, Lawrence C Layman, Ali Eroglu. Retrieval of rhesus monkey (Macaca mulatta) oocytes by ultrasound-guided needle aspiration: problems and solutions. Mol Reprod Dev. 2009, 76:890-896.
    20. Wei Si, James D Benson, Hongsheng Men, John K Critser. Osmotic tolerance limits and effects of cryoprotectants on the motility, plasma membrane integrity and acrosomal integrity of rat sperm. Cryobiology. 2006. 53:336-48.
    21. Wei Si, Hong Wang, Catherine Reid, Thomas B Hildebrandt, Weizhi Ji. Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm. Am J Primatol. 2006. 68:103-108.
    22. Wei Si, Thomas B Hildebrandt, Catherine Reid, Ronald Krieg, Weizhi Ji, Mirja Fassbender, Robert Hermes. The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant. Theriogenology. 2006. 65:788-798.
    23. Wei Si, Ping Zheng, Yahui Li, Andras Dinnyes, Weizhi Ji. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (macaca mulatta) sperm. Am J Primatol, 2004. 62:301-306.
    24. Wei Si, Ping Zheng, Xianghui Tang, Xiechao He, Hong Wang, Barry DBavister, Weizhi Ji. Cryopreservation of rhesus monkey (macaca mulatta) spermatozoa and functional assessment by in vitro fertilization. Cryobiology. 2000. 41: 232-240.
    25. 付旭锋,陈冰冰,严亚萍,王宏,王峻峰,郑冰蓉,司维*。动物模型在间充质干细胞治疗慢性肝病临床前研究的应用,中国实验动物学报,2017,25:102-106.
    26. 付旭锋,刘平,陈冰冰,焦扬,司维*,郑冰蓉*。间充质干细胞冻存方法的研究进展,云南大学学报自然科学版, 2016, 38:652-660。

    六、专著

    季维智 主编,杨世华,司维,牛昱宇,副主编: 《猕猴繁殖生物学》,科学出版社. 2013

    七、授权发明专利

    1. 牛昱宇,王宏,司维,郭祥玉,司晨洋,王芳,张琼书,施桂英,树鼩非酒精单纯性脂肪肝动物模型的构建方法,2012.10.3-2032.4.12,中国,CN201210106203.X。
    2. 王宏,牛昱宇,司维,纪少珲,赵博,季维智,猕猴肝损伤动物模型构建方法,2010.11.10-2030.6.13,中国,CN201010200583.4。
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