• 司维(Wei Si)

    博士, 教授,硕士生导师

    Email: siw@lpbr.cn

    一、受教育及工作经历 Education and Working Experience

    1993年-1997年,云南大学生物系,生物学专业,获生物学学士学位

    1997年-2004年,中科院昆明动物研究所,动物学专业,生殖生物学研究方向获动物学博士学位

    2004年2月 – 2005年2月,中科院昆明动物研究所,实习研究员

    2005年2月 –2007年11月,美国University of Missouri-Columbia,博士后

    2007年11月-2008年11月,美国 Medical College of Georgia,博士后

    2008年12月-2014年12月,中科院昆明动物研究所,副研究员

    2009年7-9月,德国Institute for Zoo and Wildlife Research,Max Planck Society访问学者

    2011年12月至今,云南中科灵长类生物医学重点实验室,研究员

    2014年12月至今,昆明理工大学灵长类转化医学研究院,教授

     

    1. 1993-1997, Bachelor's Degree, Department of Biology, Yunnan University.
    2. 1997-2004, PhD, Kunming Institute of Zoology, Chinese Academy of Sciences, majoring in zoology.
    3. 2004 - 2005, Research assistant, Kunming Institute of Zoology, Chinese Academy of Sciences.
    4. 2005- 2007, Postdoctoral, University of Missouri-Columbia, USA.
    5. 2007- 2008, Postdoctoral, Medical College of Georgia, USA.
    6. , Associate Professor , Kunming Institute of Zoology, Chinese Academy of Sciences.
    7. Since 2014, Professor, Institute of Primate Translational Medicine, Kunming University of Technology.

    二、主要研究方向 Research Interests

    人类疾病的灵长类动物模型建立和细胞/基因治疗的有效性和安全性评价的临床前研究,人类疾病与肠道菌群之间关系的动物实验模型,灵长类种质资源(精子、卵母细胞和胚胎)和干细胞细胞的超低温保存的冷冻损伤机制和提高保存效率的对策;灵长类发育过程中细胞分化的基因组表达和甲基化调控。

     

    Establishment of primate animal models of human diseases and pre-clinical studies on effectiveness and safety of cell and gene therapy; Relationship between diseases and intestinal microorganisms; Mechanism of freezing injury in cryopreservation of germ cells and stem cell cells; Regulation of genomic expression during primate embryonic development.

    四、承担科研项目情况 Research Projects and Grants

    1. 国家自然科学基金面上项目:猕猴肝纤维化疾病模型间充质干细胞移植治疗的细胞示踪以及移植细胞基因表达调控的临床前研究(31872973),2019-2022年,58万,主持
    2. 国家自然科学基金地区基金项目:灵长类动物早期着床胚胎三胚层分化和器官发生基因组表达调控的研究(31660346),2017-2020年,43万,主持
    3. 国家重点研发计划“干细胞及转化研究”试点专项:靶向基因编辑建立神经系统疾病猴模型及干细胞治疗研究(子课题2016YFA0101400),2017-2020年,320万,骨干
    4. 云南省科技计划项目:人脐带MSC临床适用性关键技术建立及其在猕猴肝纤移植治疗中的应用(2018ZF7-05),2018-2020, 140万,骨干
    5. 国家重大科学研究计划重大科学问题目标导向项目 “细胞多能性和人类重大疾病的猴模型研究” 子课题:猕猴肝损伤动物模型(2012CBA01305),2012-2016年,129.5万,主持
    6. 云南省中青年学术和技术带头人后备人才,2012-2015年,12万,主持
    1. The National Natural Science Foundation of China (31660346), 2017-2020, ¥430,000, PI.
    2. The National Natural Science Foundation of China (31660346), 2019-2022, ¥580,000, PI.
    3. The National Key Research and Development Program of China (2016YFA0101403), 2016-2020, ¥3,200,000, Co-PI.
    4. 4.云南省科技计划项目:人脐带MSC临床适用性关键技术建立及其在猕猴肝纤移植治疗中的应用(2018ZF7-05),2018-2020, 140万,骨干
    5. Yunnan Science and Technology Project (2018ZF7-05), 2018-2020, ¥1,400,000, Co-PI.
    6. 5.国家重大科学研究计划重大科学问题目标导向项目 “细胞多能性和人类重大疾病的猴模型研究” 子课题:猕猴肝损伤动物模型(2012CBA01305),2012-2016年,129.5万,主持
    7. The National Basic Research Program of China (2012CBA01305), 2012-2016, ¥1,295,000, PI.
    8. Yunnan young leaders in academic and technical talents project (2012HB040), 2012-2015, ¥120,000, PI.

    五、获奖情况 Awards

    1. 司维,季维智,李亚辉,郑萍,牛昱宇,和协超,纪少珲。生物医学实验动物精子超低温冷冻保存,云南省自然科学奖,三等奖,2012。
    2. 王宏,季维智,严晔,牛昱宇,司维,纪少珲,马原野。标准化灵长类实验动物国际认证建设与应用,云南省科学技术进步奖,三等奖,2015。
    3. 季维智,牛昱宇,陈永昌,司维,王宏,康宇,司晨洋。非人灵长类基因编辑与神经系统疾病模型研究,云南省自然科学奖,一等奖,2017。
    1. Si Wei, Ji Weizhi, Li Yahui, Zheng Ping, Niu Yuyu, He Xiechao, Ji Shaohui. Yunnan Natural Science Award, Third Prize, 2012.
    2. Wang Hong, Ji Weizhi, Yan Ye, Niu Yuyu, Si Wei, Ji Shaohui, Ma Yuanye. Yunnan Science and Technology Progress Award, Third Prize, 2015.
    3. Ji Weizhi, Niu Yuyu, Chen Yongchang, Si Wei, Wang Hong, Kang Yu, Si Chenyang. Yunnan Natural Science Award, First Prize, 2017.

    六、代表性论文和专著 Publications

    1. Wang H#, Yan Y#, Yi X#, Duan Y, Wang J, Li S, Luo L, Huang T, Inglis B, Li X, Ji W, Tan T*, Si W*. Histopathological features and composition of gut microbiota in rhesus monkey of alcoholic liver disease. Frontier in Microbiology. 2019, 10: 165.
    2. Wang Y#, Wang H#, Zhang Y#, Du Z#, Si W#, Fan S, Qin D, Wang M, Duan Y, Li L, Jiao Y, Li Y, Wang Q, Shi Q, Wu X*, Xie W*. Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis. Molecular Cell. 2019, 73:547-561.
    3. Yan L#, Jiang B#, Niu Y#, Wang H, Li E, Yan Y, Sun H, Duan Y, Chang S, Chen G, Ji W*, Xu RH*, Si W*. Intrathecal delivery of human ESC-derived mesenchymal stem cell spheres promotes recovery of a primate multiple sclerosis model. Cell Death Discov. 2018, 5:28.
    4. Jiao Y, Zhao H, Chen G, Sang X, Yang L, Hou Z*, Si W*, Zheng B*. Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs. Stem Cells Int. 2018:5912194.
    5.  Fu X#, Jiang B#, Zheng B#, Yan Y, Wang J, Duan Y, Li S, Yan L, Wang H, Chen B, Sang X, Ji W*, Xu R*, Si W*. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse. PeerJ. 2018, 6:e4336.
    6. Fu X#, Yan Y#, Li S, Wang J, Jiang B, Wang H, Duan Y, Tan T, Gao F, Gong D, Niu Y, Ji W*, Zheng B*, Si W*. Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression. Stem Cells Int. 2017:3893691.
    7. Chen B#, Li S#, Yan Y, Duan Y, Chang S, Wang H, Ji W*, Wu X*, Si W*. Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels - CatSper and Hv1. Theriogenology. 2017, 104:37-42.
    8. Gao F, Niu Y, Sun YE, Lu H, Chen Y, Li S, Kang Y, Luo Y, Si C, Yu J, Li C, Sun N, Si W, Wang H, Ji W, Tan T. De novo DNA methylation during monkey pre-implantation embryogenesis. Cell Res. 2017, 27:526-539.
    9. Park JW, Yan L, Stoddard C, Wang X, Yue Z, Crandall L, Robinson T, Chang Y, Denton K, Li E, Jiang B, Zhang Z, Martins-Taylor K, Yee SP, Nie H, Gu F, Si W, Xie T, Yue L, Xu RH. Recapitulating and Correcting Marfan Syndrome in a Cellular Model. Int J Biol Sci. 2017, 13:588-603.
    10. Yan Y, Ao L, Wang H, Duan Y, Chang S, Chen B, Zhi D, Li S, Niu Y, Ji W, Si W*. Optimization of cryopreservation of cyomolgus macaque (Macaca fascicularis) sperm with commercial egg yolk free SpermCryo All-round medium. J Am Assoc Lab Anim Sci. 2016, 55:744-748.
    11. Wang H, Tan T, Wang J, Niu Y, Yan Y, Guo X, Kang Y, Duan Y, Chang S, Liao J, Si C, Ji W*, Si W*. Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis. Sci Rep. 2015;5:15019.
    12. Niu Y, Guo X, Chen Y, Wang C, Gao J, Yang W, Kang Y, Si W, Wang H, Yang S, Li S, Ji W, Li X. Early Parkinson's disease symptoms in α-synuclein transgenic monkeys. Hum Mol Genet. 2015, 24: 2308–2317. 
    13.  Zhou T, Wang G, Chen M, Zhang M, Guo Y, Yu C, Zhou Z, Si W, Sha J, Guo X. Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition. Proteomics. 2015, 15:1564-73.
    14. Liu H, Chen Y, Niu Y, Zhang K, Kang Y, Ge W, Liu X, Zhao E, Wang C, Lin S, Jing B, Si C, Lin Q, Chen X, Lin H, Pu X, Wang Y, Qin B, Wang F, Wang H, Si W, Zhou J, Tan T, Li T, Ji S, Xue Z, Luo Y, Cheng L, Zhou Q, Li S, Sun YE, Ji W. TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys. Cell Stem Cell. 2014, 14:323-8.
    15. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X, Ji W, Sha J. Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Cell. 2014, 156:836-43.
    16. Tan T, Lu B, Zhang J, Niu Y, Si W, Wei Q, Ji W. Notch1 signaling antagonizes transforming growth factor-β pathway and induces apoptosis in rabbit trophoblast stem cells. Stem Cells Dev. 2014, 23:813-22.
    17. He H, Teng H, Zhou T, Guo Y, Wang G, Lin M, Sun Y, Si W, Zhou Z, Guo X, Huo R. Unravelling the proteome of adult rhesus monkey ovaries. Mol Biosyst. 2014,10:653-62.
    18. Chen Y, Niu Y, Yang S, He X, Ji S, Si W, Tang X, Xie Y, Wang H, Lu Y, Zhou Q, Ji W. The available time window for embryo transfer in the rhesus monkey (Macaca mulatta). Am J Primatol. 2012, 74:165-73.
    19. Yang S, Ping S, Ji S, Lu Y, Niu Y, Wang H,Ji W, Si W*. The positive effects of seminal plasma during freezing process on cryosurvival of sperm with poor freezability in Rhesus Macaque (Macaca mulatta). J Reprod Dev. 2011, 57:737-43.
    20. Yang S, Ping S, Si W*, He X, Wang X, Lu Y, Ji S, Niu Y, Ji W. Optimize Ethylene Glycol Concentrations, Freezing Rates and Holding Times in Liquid Nitrogen Vapor for Sperm Cryopreservation of Rhesus Macaque (Macaca mulatta). J Vet Med Sci. 2011, 73:717-723.
    21. Men H, Zhao C, Si W, Murphy CN, Spate L, Liu Y, Walters EM, Samuel MS, Prather RS, Critser JK. Birth of piglets from in vitro-produced, zona-intact porcine embryos vitrified in a closed system. Theriogenology. 2011, 76:280-289.
    22. Ji S, Wang X, Shu J, Sun A, Si W, Guo X, Zhao B, Ji W, Jin L. In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta). In Vitro Cell Dev Biol Anim. 2011, 47:383-390.
    23. Si W, Lu Y, He X, Ji S, Niu Y, Tan T, Ji W. Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm. Theriogenology. 2010, 74:1431–1438.
    24. Niu Y, Yu Y, Bernat A, Yang S, He X, Guo X, Chen D, Chen Y, Ji S, Si W, Lv Y, Tan T, Wei Q, Wang H, Shi L, Guan J, Zhu X, Afanassieff M, Savatier P, Zhang K, Zhou Q, Ji W. Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector. Proc Natl Acad Sci U S A. 2010, 107:17663-17667.
    25. Si W, Men H, Benson JD, Critser JK. Osmotic characteristics and fertility of murine spermatozoa collected in different solutions. Reproduction. 2009, 137:215-223.
    26. Rodriguez NA, Si W, Emmi AM, Layman LC, Eroglu A. Retrieval of rhesus monkey (Macaca mulatta) oocytes by ultrasound-guided needle aspiration: problems and solutions. Mol Reprod Dev. 2009, 76:890-896.
    27. Si W, Benson JD, Men H, Critser JK. Osmotic tolerance limits and effects of cryoprotectants on the motility, plasma membrane integrity and acrosomal integrity of rat sperm. Cryobiology. 2006. 53:336-48.
    28. Si W, Wang H, Reid C, Hildebrandt TB, Ji W. Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm. Am J Primatol. 2006. 68:103-108.
    29. Si W, Hildebrandt TB, Reid C, Krieg R, Ji W, Fassbender M, Hermes R. The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant. Theriogenology. 2006. 65:788-798.
    30. Si W, Zheng P, Li Y, Dinnyes A, Ji W. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (macaca mulatta) sperm. Am J Primatol, 2004. 62:301-306.
    31. Zheng P, Si W, Bavister BD, Yang J, Ding C, Ji W. 17Beta-estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys. Hum Reprod. 2003. 18:2137-2144.
    32. Li Y, Si W, Zhang X, Dinnyes A, Ji W. 2003. Effect of amino acids on cryopreservation of cynomolgus monkey (macaca fascicularis) sperm. Am J Primatol. 59:159-165.
    33. Zheng P, Si W, Wang H, Zou R, Bavister BD, Ji W. Effect of age and breeding season on the developmental capacity of oocytes from unstimulated and follicle-stimulating hormone-stimulated rhesus monkeys. Biol Reprod. 2001. 64:1417-1421.
    34. Si W, Zheng P, Tang X, He X, Wang H, Bavister BD, Ji W. Cryopreservation of rhesus monkey (macaca mulatta) spermatozoa and functional assessment by in vitro fertilization. Cryobiology. 2000. 41: 232-240
    35. 付旭锋陈冰冰,严亚萍,王宏,王峻峰,郑冰蓉,司维*。动物模型在间充质干细胞治疗慢性肝病临床前研究的应用,中国实验动物学报,2017,25:102-106.
    36. 付旭锋刘平陈冰冰焦扬司维*,郑冰蓉*。间充质干细胞冻存方法的研究进展,云南大学学报自然科学版, 2016, 38:652-660。

     

    专著:
    季维智 主编,杨世华,司维,牛昱宇,副主编: 《猕猴繁殖生物学》,科学出版社. 2013

     

    授权发明专利:

    1. 牛昱宇,王宏,司维,郭祥玉,司晨洋,王芳,张琼书,施桂英,树鼩非酒精单纯性脂肪肝动物模型的构建方法,2012.10.3-2032.4.12,中国,CN201210106203.X。

    2. 王宏,牛昱宇,司维,纪少珲,赵博,季维智,猕猴肝损伤动物模型构建方法,2010.11.10-2030.6.13,中国,CN201010200583.4。

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