司维(Wei Si)

副院长
教授，博士生导师

Email: siw@lpbr.cn

一、受教育及工作经历 Education and Working Experience

1993年9月－1997年7月，云南大学生物系，生物学专业，获生物学学士学位
1997年9月－2004年2月，中科院昆明动物研究所，动物学专业，生殖生物学研究方向，获动物学博士学位
2004年2月－2005年2月，中科院昆明动物研究所，实习研究员

2005年2月－2007年11月，美国University of Missouri-Columbia，博士后
2007年11月－2008年11月，美国 Medical College of Georgia，博士后
2008年12月－2014年12月，中科院昆明动物研究所，副研究员
2014年12月至今，昆明理工大学灵长类转化医学研究院，教授

1993 - 1997, Bachelor's Degree, Department of Biology, Yunnan University.

1997 - 2004, PhD, Kunming Institute of Zoology, Chinese Academy of Sciences.

2004 - 2005, Research assistant, Kunming Institute of Zoology, Chinese Academy of Sciences.

2005 - 2007, Postdoctoral, University of Missouri-Columbia, USA.

2007 - 2008, Postdoctoral, Medical College of Georgia, USA.

Since 2014, Professor, Institute of Primate Translational Medicine, Kunming University of Technology.

二、主要研究方向 Research Interests

研究方向包括：
1.人类疾病的灵长类动物模型建立和细胞/基因治疗的有效性和安全性评价的临床前研究；

2.人类疾病与肠道菌群之间关系的动物实验模型；

3.灵长类种质资源（精子、卵母细胞和胚胎）和干细胞细胞的超低温保存的冷冻损伤机制。团队建立了高效的灵长类种质资源及干细胞保存体系，开发了多种灵长类动物疾病模型(关节炎、糖尿病、肝纤维化和帕金森等)平台，并开展多项人MSC、iPSC等干细胞移植治疗的临床前研究。

Establishment of nonhuman primate models for human diseases and pre-clinical studies on effecacy and safety of cell and gene therapy; Relationship between diseases and intestinal microorganisms; Mechanism of freezing injury to germ cells and stem cell cells during cryopreservation.
Our research interests include:

1. Generation of primate animal models of human diseases and preclinical studies on the efficacy and safety of cell or gene therapy;

2. Diseases and intestinal microbiota;

3. Cryodamage mechanism of primate germplasm resources (sperm, oocyte and embryo) and stem cell cryopreservation. Our team has established efficient methods for germplasm and stem cell cryopreservation, and developed various primate animal models (arthritis, diabetes, liver fibrosis and Parkinson) and carried out pre clinical studies on stem cell transplantation of MSCs and iPSCs.

三、承担科研项目情况 Research Projects and Grants

国家重点研发计划项目：发育代谢复杂疾病灵长类动物模型的创制与临床前研究，2018YFA0801400， 2019-2024，7050万元，首席科学家。
国家自然科学基金面上项目：猕猴肝纤维化疾病模型间充质干细胞移植治疗的细胞示踪以及移植细胞基因表达调控的临床前研究（31872973），2019-2022年，58万，主持。

National Key Research and Development Project （2018YFA0801400）, 2019-2024，￥70,050,000, Chief scientist.
National Natural Science Foundation of China (31872973), 2019-2022, ￥580,000, PI.

四、获奖情况 Awards

季维智，牛昱宇，陈永昌，司维，王宏，康宇，司晨洋。非人灵长类基因编辑与神经系统疾病模型研究，云南省自然科学奖，一等奖，2017。
王宏，季维智，严晔，牛昱宇，司维，纪少珲，马原野。标准化灵长类实验动物国际认证建设与应用，云南省科学技术进步奖，三等奖，2015。
司维，季维智，李亚辉，郑萍，牛昱宇，和协超，纪少珲。生物医学实验动物精子超低温冷冻保存，云南省自然科学奖，三等奖，2012。
郑冰蓉，司维，付旭峰。基因序列变异及干细胞干预对复杂疾病的作用影响研究，云南省自然科学奖，三等奖，2012。

五、代表性论文和专著 Publications

Yu Kang#, Shaoxing Dai#, Yuqiang Zeng#, Fang Wang#, Pengpeng Yang, Zhaohui Yang, Youwei Pu1,3, Zifan Li, Xinglong Chen1, Baohong Tian, Wei Si, Weizhi Ji*, Yuyu Niu*. Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis. Science Advances. 2022.7 8(29)：1-11 [IF: 14.957]
Li S#, Wang J#, Jiang B#, Jiang J#, Luo L, Zheng B*, Si W*. Mesenchymal stem cells derived from different perinatal tissues donated by same donors manifest variant performance on the acute liver failure model in mouse. Stem Cell Research & Therapy. 2022.6 2022, 13: 231. [IF: 6.832]
Yaping Yan #, Junfeng Wang # , Shuai Qiu, Yanchao Duan , Wei Si *.The Lumenal Microbiota Varies Biogeographically in the Gastrointestinal Tract of Rhesus Macaques.Microbiol Spectr.e0034322.2022.5.[IF: 7.171]
Yuan H^#, Sun J^#, Wang S^#, Xiang Z, Yang F, Yan Y, Duan Y, Li L, Wu X^*, Si W^*. Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey. J Cell Mol Med. 2022 Feb 1. doi: 10.1111/jcmm.17197. Online ahead of print.
Yan Y^#, Ren S^#, Duan Y, Lu C, Niu Y, Wang Z, Inglis B, Ji W^*, Zheng Y^*, Si W^*. Gut microbiota and metabolites of α-synuclein transgenic monkey models with early stage of Parkinson's disease. NPJ Biofilms Microbiomes. 2021 Sep 2;7(1):69.
Yan Y^#, Yi X^#, Duan Y^#, Jiang B, Huang T, Inglis BM, Zheng B^*, Si W^*. Alteration of the gut microbiota in rhesus monkey with spontaneous osteoarthritis. BMC Microbiol. 2021, 21(1):328.
Chen B^#, Wang S^#, Inglis BM^#, Ding H, Suo A, Qiu S, Duan Y, Li X, Li S, Sun WQ^*, Si W^*. Improving Sperm Cryopreservation With Type III Antifreeze Protein: Proteomic Profiling of Cynomolgus Macaque (Macaca fascicularis) Sperm. Front Physiol. 2021, 12:719346.
Wang J^#, Fu X^#, Yan Y, Li S, Duan Y, Marie Inglis B, Si W^*, Zheng B^*. In vitro differentiation of rhesus macaque bone marrow- and adipose tissue-derived MSCs into hepatocyte-like cells. Experimental and Therapeutic Medicine. 2020，20:251-260.
Fu X^#*, Xu B^#, Jiang J^#, Du X, Yu X, Yan Y, Li S, Inglis BM, Ma H, Wang H, Pei X^*, Si W^*. Effects of cryopreservation and long-term culture on biological characteristics and proteomic profiles of human umbilical cord-derived mesenchymal stem cells. Clinical Proteomics. 2020,17:15.
Jiang B^#, Fu X^#, Yan L^#, Li S^#, Zhao D, Wang X, Duan Y, Yan Y, Li E, Wu K, Inglis B, Ji W*, Xu RH*, Si W*. Transplantation of human ESC-derived mesenchymal stem cell spheroids ameliorates spontaneous osteoarthritis in rhesus macaques. Theranostics. 2019, 9: 6587-6600.
Wang S^#, Duan Y^#, Yan Y, Adar C, Braslavsky I, Chen B, Huang T, Qiu S, Li X, Inglis BM, Ji W, Si W*. Improvement of sperm cryo-survival of cynomolgus macaque (Macaca fascicularis) by commercial egg-yolk–free freezing medium with type III antifreeze protein. Animal Reproduction Science. 2019, 210:106177.
Li S^#, Ao L^#, Yan Y^#, Jiang J, Chen B, Duan Y, Shen F, Chen J, Inglis B, Ni R, Ji W*, Si W*. Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial. Clinical Proteomics. 2019, 16:24.
Wang H^#, Yan Y^#, Yi X^#, Duan Y, Wang J, Li S, Luo L, Huang T, Inglis B, Li X, Ji W, Tan T*, Si W*. Histopathological features and composition of gut microbiota in rhesus monkey of alcoholic liver disease. Frontiers in Microbiology. 2019, 10: 165.
Wang Y^#, Wang H^#, Zhang Y^#, Du Z^#, Si W^#, Fan S, Qin D, Wang M, Duan Y, Li L, Jiao Y, Li Y, Wang Q, Shi Q, Wu X*, Xie W*. Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis. Molecular Cell. 2019, 73:547-561.
Yan L^#, Jiang B^#, Niu Y^#, Wang H, Li E, Yan Y, Sun H, Duan Y, Chang S, Chen G, Ji W*, Xu RH*, Si W*. Intrathecal delivery of human ESC-derived mesenchymal stem cell spheres promotes recovery of a primate multiple sclerosis model. Cell Death Discov. 2018, 5:28.
Jiao Y, Zhao H, Chen G, Sang X, Yang L, Hou Z*, Si W*, Zheng B*. Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs. Stem Cells Int. 2018:5912194.
Fu X^#, Jiang B^#, Zheng B^#, Yan Y, Wang J, Duan Y, Li S, Yan L, Wang H, Chen B, Sang X, Ji W*, Xu R*, Si W*. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse. PeerJ. 2018, 6:e4336.
Fu X^#, Yan Y^#, Li S, Wang J, Jiang B, Wang H, Duan Y, Tan T, Gao F, Gong D, Niu Y, Ji W*, Zheng B*, Si W*. Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression. Stem Cells Int. 2017:3893691.
Chen B^#, Li S^#, Yan Y, Duan Y, Chang S, Wang H, Ji W*, Wu X*, Si W*. Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels - CatSper and Hv1. Theriogenology. 2017, 104:37-42.
Yan Y^#, Ao L^#, Wang H, Duan Y, Chang S, Chen B, Zhi D, Li S, Niu Y, Ji W, Si W*. Optimization of cryopreservation of cyomolgus macaque (Macaca fascicularis) sperm with commercial egg yolk free SpermCryo All-round medium. J Am Assoc Lab Anim Sci. 2016, 55:744-748.
Wang H, Tan T, Wang J, Niu Y, Yan Y, Guo X, Kang Y, Duan Y, Chang S, Liao J, Si C, Ji W*, Si W*. Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis. Sci Rep. 2015;5:15019.

六、专著

季维智主编，杨世华，司维，牛昱宇，副主编: 《猕猴繁殖生物学》，科学出版社. 2013

七、授权发明专利

牛昱宇，王宏，司维，郭祥玉，司晨洋，王芳，张琼书，施桂英，树鼩非酒精单纯性脂肪肝动物模型的构建方法，2012.10.3-2032.4.12，中国，CN201210106203.X。
王宏，牛昱宇，司维，纪少珲，赵博，季维智，猕猴肝损伤动物模型构建方法，2010.11.10-2030.6.13，中国，CN201010200583.4。