• 王宏(Wang Hong)

    高级兽医师

    DVM, Senior veterinarian, Associate researcher

    Email:wangh@lpbr.cn

    一、受教育及工作经历 EDUCATION AND WORK EXPERIENCE

    1996年9月-2000年7月,湖北农学院(现长江大学)动物科学系,农学学士学位

    2000年8月-2001年8月,湖北武汉东西湖畜牧兽医研究所,兽医

    2001年9月-2004年6月,福建农林大学动物科学学院,农学硕士学位

    2004年7月-2014年12月,中国科学院昆明动物研究所,助理研究员

    2006年5月-2007年7月,美国Oregon National Primate Research Center, Oregon Health & Science University,兽医学访问学者

    2012年9月-2015年8月,昆明亚灵生物科技有限公司,高级兽医师

    2012年9月至今,云南中科灵长类生物医学重点实验室,高级兽医师

    2016年1月至今,中国农业大学动物医学院,临床兽医博士在读

    2016年4月至今,昆明理工大学灵长类转化医学研究院,副研究员、高级兽医师

     

    EDUCATION

    5/2006-6/2007 Visiting scholar in Primate Medicine, Animal Resource Division of Oregon National Primate Research Center, Oregon Health & Science University, Oregon, USA.

    1/2016-6/2019 Doctor Degree in Veterinary Medicine, Animal Medicine College of China Agriculture University, Beijing, China.

    9/2001-6/2004 Master Degree in Animal Genetics and Reproduction, Animal Science College of Fujian Agricultural and Forestry University, Fujian, China.

    9/1996-6/2000 Bachelor Degree in Veterinary Medicine, Animal Husbandry and Veterinary Medicine College of Hubei Agriculture University, Hubei, China.

     

    WORK EXPERIENCE

    4/2016 to the present Associate researcher, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.

    9/2012 to the present Senior veterinarian, Yunnan Key Laboratory of Primate Biomedicine Research.

    9/2012-8/2015 Senior veterinarian, Kunming Biomed International.

    5/2004-12/2014 Veterinary and assistant researcher, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences.

    8/2000-8/2001 Veterinary in living animals, Wuhan DONGXI-Lake Institute of Animal Science and Veterinary, Hubei, China.

     

    QUALIFICATION SUMMARY:

    • Self motivated with a strong positive attitude, sense of responsibility and flexibility at work.
    • More than 15 years experience in managing in-life activities of preclinical safety studies of pharmaceutics in non-human primates(monkey, tree shrew) and rodents (mice, rat).
    • Familiarity with non-human primates breeding, animal nutrition, anatomy, behavior observation, disease treatment, nursing care and operative surgery.
    • Familiarity with AAALAC requirements and guideline for animal use and welfare.
    • Familiar with writing and reviewing Standard Operating Procedure (SOP).
    • Outstanding skills of project management, team management and preclinical pharmacology management.
    • Familiar with CRO in protocol development, execution of studies, compilation of study results, QC of study data and responses to QA audit and managing other pharmacology and toxicology study.
    • Expert on animal room and functional design.
    • Expert on various experimental techniques in histology, pathology, molecular biology, ultrasound imaging and laboratory clinical diagnosis.
    • Proficient in technique to make animal disease model in CNS diseases and metabolic diseases.

    二、主要研究方向 MAIN RESEARCH DIRECTION

    1、实验动物标准化研究与应用

    2、灵长类动物疾病诊断与治疗

    3、人类疾病灵长类动物模型建立和评价研究

     

    • Study and application of laboratory animal standardization
    • Diagnosis and treatment of nonhuman primate diseases
    • Establishment of primate model of human disease and study on pathogenic mechanism

    三、承担或参与科研项目

    1. 国家人力资源和社会保障部“猕猴酒精性脂肪肝模型的创制”,2014-2017,主持。
    2. 云南省科技厅“云南省技术创新人才培养计划项目”,2014-2017,主持。
    3. 昆明市科技局重点项目“无特定病原体实验猕猴培育关键技术研究与应用”,2014-2016,主持。
    4. 云南省科技厅重点项目“猕猴膨化饲料生产关键技术研究与示范”,2015-2017,主持。
    5. 国家科技支撑计划项目“灵长类实验动物标准化研究与数据管理系统建设”,2007-2011,子课题负责人。
    6. 科技部重大专项“评价抗HCV化学药物疗效和安全性的动物模型”,2009-2012,第一参与人。
    7. 云南省国际合作项目“标准化灵长类实验动物国际认证建设”,2006-2009,第一参与人。
    8. 科技部973课题“肝炎等重大人类疾病的灵长类模型研制”,2012-2016,第二参与人。
    9. 科技部863课题“神经退行性疾病灵长类动物模型制备关键技术及利用”,2012-2015,第五参与人。
    10. 国家自然科学基金重点项目“帕金森氏症转基因灵长类动物模型的构建与致病机理研究”,2014-2017,第五参与人。

    四、奖励情况

    2012年,昆明市第十批中青年学术和技术后备人选

    2013年,昆明市科技进步一等奖

    2014年,云南省技术创新人才

    2015年,云南省科技进步三等奖

    2016年,昆明市第十四批中青年学术带头人

    2017年,昆明市科技进步三等奖

    五、授权和申请专利

    1. 王宏、牛昱宇、司维、纪少珲、赵博、季维智,猕猴肝损伤动物模型构建方法,授权号:ZL201010200583.4
    2. 王宏、季维智、严晔,通用型灵长类实验动物的标准化饲养装置,授权号:ZL201020235892.0
    3. 牛昱宇、王宏、司维、郭祥玉、司晨洋、王芳、张琼书、施桂英,树鼩非酒精单纯性脂肪肝动物模型的构建方法,授权号:ZL201210106203.X
    4. 王宏、牛昱宇,一种实验用树鼩固定装置,授权号:ZL201120317195.4
    5. 王宏、季维智、牛昱宇、司维,一种实验用灵长类动物多功能转移笼,授权号:ZL20152061781.3
    6. 王宏、韦秋奖,一种无特定病原体级实验动物出口检疫期的养殖方法,申请号:201510518168.9
    7. 司维、王宏、严亚萍、王峻峰、常绍辉、段艳超、牛昱宇、季维智,黄曲霉素诱导猕猴肝纤维化模型的方法,申请号:201610692028.5

    六、著作

    《猕猴繁殖生物学》,科学出版社,负责编写第十三、十四、十五、十六和十七章共五章内容,2013年。

     

    七、代表性论文 PUBLICATIONS

    1.  Lei Shi, Xin Luo, Jin Jiang, Yongchang Chen, Cirong Liu, Ting Hu, Min Li, Qiang Lin, Yanjiao Li, Jun Huang, Hong Wang, Yuyu Niu, Yundi Shi, Martin Styner, Jianhong Wang, Yi Lu, Xuejin Sun, Hualin Yu, Weizhi Ji,Bing Su. Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development. National Science Review,2019, DOI: 10.1093/nsr/nwz043/5420749
    2. Hong Wang, Yaping Yan,Xiaoyan Yi,Yanchao Duan, Junfeng Wang, Shanshan Li, Lilin Luo, Tianzhuang Huang,Briauna Inglis, Xi Li, Weizhi Ji, Tao Tan, Wei Si. Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease. Frontiers in Microbiology,2019, doi.org/10.3389/fmicb.2019.00165.
    3. Yiqiang Cui, Yuyu Niu, Jiankui Zhou,Yongchang Chen, Yiwei Cheng, Shangang Li,Zongyong Ai, Chu Chu, Hong Wang, Bo Zheng,Xuejin Chen, Jiahao Sha, Xuejiang Guo,Xingxu Huang, Weizhi Ji. Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination. Cell Research, 2018,1:1-4.
    4. Fu X, Jiang B, Zheng B, Yan Y, Wang J, Duan Y, Li S, Yan L, Wang H, Chen B, Sang X, Ji W, Xu R, Si W*. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse. Peer J, 2018, 6:e4336.
    5. Fu X, Yan Y, Li S, Wang J, Jiang B, Wang H, Duan Y, Tan T, Gao F, Gong D, Niu Y, Ji W, Zheng B, Si W*. Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression. Stem Cells Int. 2017:3893691.
    6. Gao F, Niu Y, Sun YE, Lu H, Chen Y, Li S, Kang Y, Luo Y, Si C, Yu J, Li C, Sun N, Si W, Wang H, Ji W, Tan T. De novo DNA methylation during monkey pre-implantation embryogenesis. Cell Res, 2017, 27:526-539.
    7. Bingbing Chen, Shanshan Li, Yaping Yan, Yanchao Duan,Shaohui Chang, Hong Wang, Weizhi Ji, Xin Wu, Wei Si. Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels d CatSper and Hv1. Theriogenology, 2017,104: 37-42.
    8. Wang Hong, Chen Zhigang, Chen Lixian, Zong Faliang, Li Heling. Survey and analysis of common diseases of laboratory monkeys,taking Kunming area as an example. Animal Husbandry and Feed Science, 2017,9(5):289-292.
    9. Yan Yaping, Ao Lei, Wang Hong, Duan Yanchao, Chang Shaohui, Chen Bingbing, Zhi Dalong, Li Shanshan, Niu Yuyu, Ji Weizhi, Si Wei. Cryopreservation of Cynomolgus Macaque (Macacafascicularis) Sperm by Using a Commercial Egg-Yolk–Free Freezing Medium. Journal of the American Association for Laboratory Animal Science. 2016, 55:744-748.
    10. Hong Wang, Tao Tan, Junfeng Wang, Yuyu Niu, Yaping Yan, Xiangyu Guo,Yu Kang, Yanchao Duan, Shaohui Chang, Jianpeng Liao, Chenyang Si, Weizhi Ji & Wei Si. Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis. Scientific Reports, 2015,5:15019. doi: 10.1038/srep15019.
    11. Hong Wang, Decheng Wang, Lixian Chen, Heling Li, Zhigang Chen. A case of oral cavity squamous cell carcinoma in an adult female rhesus monkey(macaca mulatta). International Journal of Advances In Case Reports, 2017;4(1):5-8.
    12. Zongyong Ai, Zheng Xiang, Yuemin Li, Guoku Liu, Hong Wang,Yun Zheng, Xiaoyan Qiu, Shumei Zhao, Xiaoqing Zhu, Yanhua Li, Weizhi Ji, Tianqing Li. Conversion of monkey fibroblasts to transplantable telencephalic neuroepithelial stem cells. Biomaterials, 2016,77:53-65.
    13. Yuyu Niu, Xiangyu Guo, Yongchang Chen, Chuan-En Wang,Jinquan Gao, Weili Yang, Yu Kang, Wei Si, Hong Wang,Shang-Hsun Yang, Shihua Li, Weizhi Ji, Xiao-Jiang Li. Early Parkinson’s disease symptoms in α-synuclein transgenic monkeys. Human Molecular Genetics, 2015, 24(8): 2308–2317.
    14. Hong Wang, Si Wei, Zhou Yin, Chen Lixian. Artificial nursing procedure establishment for infant rhesus monkey. Animal Husbandry and Feed Science,2015,7(6):381-382.
    15. Yongchang Chen, Yinghui Zheng, Yu Kang, Weili Yang, Yuyu Niu, Xiangyu Guo, Zhuchi Tu, Chenyang Si, Hong Wang, Ruxiao Xing, Xiuqiong Pu, Shang-Hsun Yang, Shihua Li, Weizhi Ji and Xiao-Jiang Li. Functional disruption of the dystrophin gene in Rhesus Monkey Using CRISPR/Cas9. Human Molecular Genetics, 2015,24(13):3764-3774.
    16. Yu Kang, Bo Zheng, Bin Shen, Yongchang Chen, Lei Wang4,Jianying Wang, Yuyu Niu, Yiqiang Cui, Jiankui Zhou, Hong Wang,Xuejiang Guo, Bian Hu, Qi Zhou, Jiahao Sha, Weizhi Ji, Xingxu Huang. CRISPR/Cas9-mediated Dax1 knockout in the monkey recapitulates human AHC-HH. Human Molecular Genetics, 2015, 24(25): 7255–7264.
    17. Hong Wang, Lixian Chen. A rare case of unusual giant gallbladder in a 24 years old female rhesus monkey(Macaca mulatta). International Journal of Advances In Case Reports, 2015,2(19):1162-1163.
    18. Bin Jiang, Zhen Xiang, Zongyong Ai, HongWang, Yanjiao Li,Weizhi Ji,Tianqing Li. Generation of cardiac spheres from primate pluripotent stem cells in a small molecule-based 3D system. Biomaterials, 2015(65): 103-114.
    19. Hong Wang, Yaping Yan, Rurun Li, Zhenqiu Chen, Yu Kang, Chenyang Si, Weizhi Ji ,Wei Si and Yuyu Niu.A timed liver tissue collection method for hepatology evaluation in tree shrew. Journal of Science,2015(7):500-504.
    20. Hailiang Liu, Yongchang Chen, Yuyu Niu, Kunshan Zhang, Yu Kang, Weihong Ge, Xiaojing Liu, Enfeng Zhao, Chencheng Wang, Shaoyun Lin, Bo Jing, Chenyang Si, Xiuqiong Pu, Yingying Wang, Binlian Qin, Fang Wang, Hong Wang, Wei Si, Jing Zhou, Tao Tan, Tianqing Li, Shaohui Ji, Zhigang Xue, Yuping Luo, Liming Cheng, Qi Zhou, Siguang Li, Yi Eve Sun, Weizhi Ji. TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys. Cell Stem Cell,2014(14):323-328.
    21. Yuyu Niu,Bin Shen,Yiqiang Cui, Yongchang Chen, Yu Kang, Xiaoyang Zhao,Wei Si, Wei Li, Andy Peng Xiang, Ye Bi, Chenyang Si, Bian Hu, Guoying Dong, Hong Wang, Zuomin Zhou, Tianqing Li, Tao Tan, Xiuqiong Pu, Fang Wang, Shaohui Ji, Qi Zhou,Xingxu Huang, Weizhi Ji, Jiahao Sha. Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Cell,2014(156):836-843.
    22. Liu Rui, Zhang Zheng, Liu Huan, Hou Panpan, Lang Jun, Wang Sheng, Yan Hongli, Wu Hongbing, Rong Mingqiang, Huang Jian, Wang Hong, Lv Longbao, Qiu Mingfeng, Ding Jiuping, Lai Ren. Human -Defensin 2 Is a Novel Opener of Ca2+-Activated Potassium Channels and Induces Vasodilation and Hypotension in Monkeys. Hypertension, 2013, 62(2): 415-425.
    23. Hong Wang, Yuyu Niu, Wei Si, Yanjiao Li, Ye Yan. Reference data of clinical chemistry, haematology and blood coagulation parameters in juvenile cynomolgus monkeys (Macaca fascicularis). Veterinarni Medicina, 2012(5):233-238.
    24. Yongchang Chen, Yuyu Niu, Shihua Yang, Shaohui Ji, Wei Si, Hong Wang, Yongqing Lu, Qi Zhou, Weizhi Ji. The available time window for embryo transfer in rhesus monkey (Macaca Mulatta). American Journal of Primatology, 2012(74):165-173.
    25. Hong Wang, Wei Si, Yuyu Niu, Lixian Chen, Ye Yan. Unusual vesical calculus in rhesus monkey (macaca mulatta). Journal of Animal and Veterinary Advances, 2012(11):1790-1792.
    26. Shihua Yang, Shuhuang Ping, Shaohui Ji, Yuyu Niu, Hong Wang, Weizhi JI, Wei Si. The positive effects of seminal plasma during the  freezing process on cryosurvival of sperm with poor freezability in the rhesus macaque. Journal of Reproduction and Development, 2011(6): 737-743.
    27. Yuyu Niu, Yang Yu, Agnieszka Bernat, Shihua Yang, Xiangyu Guo, Yongchang Chen, Shaohui Ji, Wei Si, Qiang Wei, Hong Wang, Lei Shi, Jean Guan, Xuemei Zhu, Marielle Afanassieff,Pierre Savatier, Kang Zhang, Qi Zhou, Weizhi Ji. Transgenic rhesus monkeys produced by gene transfer into early-cleavage–stage embryos using a simian immunodeficiency virus-based vector. Proc Natl Acad Sci USA(PNAS), 2010(107):17663-17667.
    28. 王宏*,陈永昌,司维,陈兴龙,周引.猕猴剖宫产及术后护理.黑龙江畜牧兽医,2015,9:168-169.
    29. 王宏*,司维,周引,陈利仙.恒河猴婴猴人工护理程序的建立.安徽农业科学,2015,43(17):159-161.
    30. 王宏*韦秋奖. 食蟹猴SPF种群建立过程中病毒抗体动态监测.中国实验动物学报,2015,23(6):573-577.
    31. 韦秋奖,王宏*. 猕猴结肠小袋纤毛虫和鞭毛虫感染调查及治疗效果研究.黑龙江畜牧兽医,2015,24:159-161.
    32. 支大龙,敖磊,王宏,季维智,司维.SpermCryo无卵黄冷冻液对食蟹猴精子冷冻保存的研究. 安徽农业科学,2015,43(13):148-151.
    33. 王宏*,韦秋奖,江海天,陈智岗.实验猕猴细菌性痢疾的治疗.中国兽医杂志,2016,52(6):97-99.
    34. 王宏*,陈智岗,宗发梁,李鹤龄. 食蟹猴直肠脱的手术治疗.中国畜牧兽医文摘,2016,32(10):206.
    35. 王宏*,陈智岗,陈利仙,宗发梁,李鹤龄.昆明地区试验猴常见疾病的调查分析. 中国畜牧兽医,2017,44(5):1557-1564.
    36. 王宏*,付学魏,陈智岗,宗发梁,李鹤龄,韦秋奖. 昆明地区恒河猴、食蟹猴种群繁殖规律和繁殖性能研究. 中国比较医学杂志,2017,27(7):34-39.
    37. 王宏*,张龙,李鹤龄,陈智岗,宗发梁. 膨化饲料对生长期猕猴增重和营养消化率的影响, 黑龙江畜牧兽医,2017,18:139-141.
    38. 付旭锋,陈冰冰,严亚萍,王宏,王峻峰,郑冰蓉,司维. 动物模型在间充质干细胞治疗慢性肝病临床前研究的应用,中国实验动物学报,2017,25:102-106.
    39. 王宏*,韦秋奖,陈智岗,周霁. 猕猴档案管理系统的开发与应用,畜牧兽医科学,2017,12:17-19.
    40. 王宏*,陈智岗,张龙,宗发梁. 猕猴大叶性肺炎的诊断与防治.黑龙江畜牧兽医,2018,8.181-182.
    41. 王宏*. 标准化猕猴养殖基地的建设与管理. 黑龙江畜牧兽医,2018,14:205-208.
    42. 李鹤龄,王宏*,陈智岗,宗发梁. 规模化猕猴养殖场的消毒管理. 上海畜牧兽医通讯,2018,4:52-53.
    43. 王宏*, 张龙, 李鹤龄, 陈智岗, 宗发梁. 膨化加工方法对猕猴饲料营养成分的影响.中国饲料,2018,15:32-35
    44. 李鹤龄,王宏*,陈智岗,宗发梁. 恒河猴和食蟹猴毛发氨基酸和微量元素测定分析. 上海畜牧兽医通讯,2018,5:8-10.
    45. 王宏,赵德明,季维智,李鹤龄.猕猴小袋纤毛虫形态学观察及分子生物学鉴定.中国兽医学报,2019,39(1):74-80.
    46. 李鹤龄,王宏*,张龙,陈智岗,宗发梁. 恒河猴脱毛症及毛发营养学分析. 上海畜牧兽医通讯,2019,1:44-46.
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