• 季维智(Weizhi Ji)

    中国科学院院士
    博士生导师,昆明理工大学特聘教授
    灵长类转化医学院 院长
    云南中科灵长类生物医学重点实验室 理事长

    E-mail:wji@lpbr.cn

    一、简历

    季维智,男,1950年生,博士生导师,中国科学院院士,昆明理工大学特聘教授,灵长类转化医学研究院院长,云南省灵长类生物医学动物重点实验室理事长,生物医学动物模型国家地方联合工程研究中心主任。“世界经济论坛全球未来理事会”理事(2016-2018),“国家干细胞研究指导协调委员会”专家,“国家重大科学研究计划生殖与发育专家组”成员(2006-2014),“国家实验动物研究委员会”专家组成员和973项目首席科学家。1978-1982年就读于云南大学生物系,1983-2012年中科院昆明动物研究所研究员,其中1987-1989年赴美国俄勒冈国家灵长类研究中心做访问学者,1995-1997年任美国威斯康星大学动物医学与生物化学系客座教授,1996-2005年任中科院昆明动物研究所所长,2005-2008年任中科院昆明灵长类研究中心主任。

    季维智院士长期坚持灵长类生殖发育生物学的研究,围绕早期胚胎发育调控,干细胞多能性和人类疾病的猴模型及致病机理等科学问题,形成了从体外受精、胚胎早期发育、基因编辑以及干细胞等系统研究体系。在优化猴生殖细胞和胚胎体外培养体系,影响胚胎发育的分子机制,干细胞的分离、自我更新和分化调控等方面都有新的发现。率先在基因编辑灵长类动物模型取得重大突破,并获得了naïve猴多能性干细。实现了灵长类(人和猴)胚胎体外延长培养,解析了灵长类胚胎发育原肠发生与发育的重要事件。在胚胎发育和干细胞的基础理论、干细胞多能性和疾病研究都有重要意义。季维智院士的系列研究成果,为我国在灵长类的研究跻身于世界先进行列发挥了积极作用。季维智院士长期为国家生殖发育、干细胞专家组服务,为中国生殖与干细胞研究作出了突出贡献,也为中国灵长类研究的国际化并跻身于世界先进行列发挥了重要作用。承担多项国家级重点研发项目,包括科技部973计划“细胞多能性和人类重大疾病的猴模型研究”、“猕猴干细胞自我更新、定向分化的分子机制”、“非人灵长类克隆及治疗性克隆的机理研究”、科技部国家科技支撑计划“人类疾病的灵长类动物模型开发和标准化研究”及科技部863计划“灵长类动物模型与安全性评价的研究与应用”等。在 Cell,Nature, Science, Cell Stem Cell,PNAS等杂志以通讯作者或第一作者发表 SCI 论文 100 余篇。其中,2014 年在 Cell 发表的基因编辑猴的论文被评价为人类疾病模型建立的里程碑性工作、入选 2014 年世界十大科技进展(MIT)、2014 年 Cell 最佳论文(Cell)、 2014 年世界最成功的 8 大事件之一(Nature)。

    二、研究成果介绍

    1.猕猴卵母细胞体外成熟和胚胎的早期发育
    季维智研究团队率先开展了优化猕猴超排体系的研究,比较了超排时间、剂量等因素后,建立了猕猴最佳体系,获得了较大数量和高质量的卵母细胞,并提高了动物的使用效率,成为领域内的标准(Theriogenology 2009,72: 243-50;Theriogenology 2008,70(1):108-14;Am J Primatol. 2007,69(11):1278-84)。在此基础上,首次建立并完善了猕猴卵母细胞的无血清培养系统。系统研究了影响卵母细胞成熟和早期胚胎发育中的细胞质因子。发现滤泡内调控卵母细胞成熟的机制及滤泡间相互调控机制的完善与猕猴年龄有关,灵长类动物卵的成熟及发育潜能受年龄影响,猕猴卵母细胞的体外成熟需要激素调控;确定了猕猴卵母细胞成熟的营养物质需求(Human Reproduction,2003,18: 2137-214;Reproduction,2002,124:515-522;Am. J. Primatol. 2001,55: 223-232;Human Reprod.2001,16:300-30;Mol. Reprod. Develop. 2001,58:348-355; Biol. Reprod. 2001, 64: 1417-142. Theriogeology 2000, 54:999-1006;Molecular Reproduction and Development 1994, 39: 25-29;Chinese Science Bulletin 1994,39: 861-865;Perspective in Primate Biology 1992,4: 63-69;Biology of Reproduction 1990, 41: 1024-1033; Endocrinology 1989, 124: 891-898)。

    2.猕猴属动物精子的冷冻保存
    灵长类动物精子的低温保存报道不多也是一个难题。精子的低温保存不仅对灵长类动物的保护有益,而且对生殖生物学的研究提供可靠的物质基础。该团队系统地从渗透剂类型、冷冻速率等方面对多种猕猴属动物开展研究,为研究灵长类动物种质资源的保存提供了理论和实践基础(J Vet Med Sci. 2011, 73(6):717-23; J Reprod Dev. 2011 57:737-43; Am. J. Primatol. 2006, 68: 103-108; Am. J. Primatol. 2006, 68: 39-49; Asian J.Androl.2005, 793): DOI:10.1111/j.1745-7262; J Androl 2005, 26: 387-395; Cell Preservation Technology 2004, 2: 29-33; Am J Primatol. 2004, 62:301-306; Am. J. Primatol. 2003, 59: 159-165; Theriogenology 2002, 58, 1253-1260; Cryobiology 2000, 41:232-240; Theriogenology 1997, 47: 1415-1424)。并对一些胚胎发育的因素做了一系列研究胚胎体外培养体系的建立(Cell Research 2010; doi: 10.1038/cr.2010.7;Cloning and Stem Cells 2007,DOI:10.1089/clo.2007.0040;Reproduction 133:947-54;Biol Reprod 2006, 10.1095/biolreprod.106.051383;Human Reproduction, 21:2564-2571)。

    3.猕猴胚胎干细胞的建立和分化研究
    建立了猕猴胚胎和iPS干细胞,率先发表了一系列干细胞的培养体系的改进、定向诱导分化等方面的有影响的结果(Stem Cells and Development 2013,doi:1089/scd.2013.0280;J Neurosci Res. 2012, 90(7):1382-91;Plos One 2011,Volume 6 | Issue 2 | e17124;Cell Research 2010,doi: 10.1038/cr.2010.7;Reproductive BioMedicine 2009,19: 426-433;Stem Cells 2009,27(8):1954-62;Cell Research 2009,19:268-270;Cell Stem Cell 2008,3:587 – 590;J. Biol. Chem. 2008,283: 35929–35940;Stem Cells 2005,25:481-489;Stem Cells 2007,10: 2004-2008;Stem Cells 2005,23:1192-1199;Archives of Andrology 2003,49: 331-336;World Journal of Gastroenterology 2003 9: 485-490)。

    4.灵长类转基因动物研究
    季维智研究团队早先就是国际上少数能成功进行猕猴转基因的实验室(Niu et al. PNAS, 2010)。季维智领导的团队,与国内有关单位合作成功运用CRISPR和TALEN技术,实现了猕猴、食蟹猴两个物种的靶向基因修饰,使我国在灵长类转基因领域处于国际领先水平。CRISPRA/Cas9研究成果于2014年2月13日在《细胞 Cell》杂志发表(Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos, Cell, Volume 156, Issue 4, 836-843),3月6日,TALENs研究结果在《细胞干细胞 Cell Stem Cell》杂志发表(TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys,Cell Stem Cell, Volume 14, Issue 3, 323-328)。

    三、近五年相关论文

     

    1. Lifeng Xiang, Yu Yin, Yun Zheng, Yanping Ma, Yonggang Li, Zhigang Zhao, Junqiang Guo, Zongyong Ai, Yuyu Niu, Kui Duan, Jingjing He, Shuchao Ren, Dan Wu, Yun Bai, Zhouchun Shang, Xi Dai, Weizhi Ji, Tianqing Li.A developmental landscape of 3D-cultured human pre-gastrulation embryos.Nature(2019) doi:10.1038/s41586-019-1875-y,12 December 2019
    2. Shanshan Li, Lei Ao, Yaping Yan, Jiang Jiang, Bingbing Chen, Yanchao Duan, Fei Shen, Jinbao Chen,Briauna Inglis, Renmin Ni, Weizhi Ji* and Wei Si*.Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial. Clin Proteom. 16, 24 (2019) doi:10.1186/s12014-019-9244-2,19 June 2019
    3. Lei Shi, Xin Luo, Jin Jiang, Yongchang Chen, Cirong Liu, Ting Hu, Min Li, Qiang Lin, Yanjiao Li, Jun Huang, Hong Wang, Yuyu Niu, Yundi Shi, Martin Styner, Jianhong Wang, Yi Lu, Xuejin Sun, Hualin Yu, Weizhi Ji, Bing Su, Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development, National Science Review, Volume 6, Issue 3, May 2019, Pages 480–493,27 March 2019
    4. Hong Wang, Yaping Yan, Xiaoyan Yi, Yanchao Duan, Junfeng Wang, Shanshan Li Lilin Luo, Tianzhuang Huang, Briauna Inglis, Xi Li, Weizhi Ji, Tao Tan* and Wei Si*. Histopathologilal Features and Composition Of Gut Microbiota in Rhesus Monkey of Alcoholic Liver disease Frontiers in Microbiology. Front Microbiol,Volume 10,165. 08 February 2019
    5. Zhang B, Zhou Z, Zhou Y, Zhang T, Ma Y, Niu Y, Ji W, Chen Y.Social-valence-related increased attention in Rett syndrome cynomolgus monkeys: An eye-tracking study. Autism Res. Volume12, Issue11,November 2019,1585-1597,07 August 2019
    6. Yuyu Niu*,Nianqin Sun,Chang Li,Ying Lei,Zhihao Huang,Jun Wu,Chenyang Si,Xi Dai,Chuanyu Liu,Longqi Liu,Su Feng,Yu Kang,Wei Si,Hong Wang,E. Zhang,Lu Zhao,Ziwei Li,Xi Luo,Guizhong Cui,Guangdun Peng,Juan Carlos Izpisúa Belmonte*,Weizhi Ji*,Tao Tan*. Dissecting primate early post-implantation development using long-term in vitro embryo culture. Science. 366(6467) :15 Nov 2019
    7. Tianqing Li*,Zongyong Ai,Weizhi Ji*.Primate stem cells: bridge the translation from basic research to clinic application. SCIENCE CHINA Life Sciences. 2019 62(1):12-21,09 August 2018
    8. Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen,Weizhi Ji and Yuyu Niu*.Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases. BMC Biotechnology. 19, 7 (2019),doi:10.1186/s12896-018-0494-2,
    9. Bin Jiang, Xufeng Fu, Li Yan, Shanshan Li, Dongliang Zhao, Xiaoyan Wang, Yanchao Duan,Yaping Yan, Enqin Li, Kunhua Wu, Briauna Marie Inglis, Weizhi Ji, Ren-He Xu, Wei Si.Transplantation of human ESC-derived mesenchymalstem cell spheroids ameliorates spontaneous osteoarthritis in rhesus macaques. Theranostics. 2019,9(22): 6587–6600. 2019 Aug 21
    10. Li Yan, Bin Jiang, Yuyu Niu, Hongxuan Wang, Enqin Li, Yaping Yan, Huiyan Sun, Yanchao Duan,Shaohui Chang, Guokai Chen, Weizhi Ji*, Ren-He Xu* and Wei Si*.Intrathecal delivery of human ESC-derived mesenchymal stem cell spheres promotes recovery of a primate multiple sclerosis model. Cell Death Discovery. 4, 89 (2018):1551-4005. 20 August 2018
    11. Shuang Wang,Lichuan Yang, Raoxian Bai, Shuaiwei Ren, Yuyu Niu, Weizhi Ji,Yuanye Ma,Yongchang Chen*.Interaction of p53 and ASPPs Regulates Rhesus Monkey Embryonic Stem Cells Conversion to Neural Fate Concomitant with Apoptosis. Cell Cycle. 17(9):1146-1153, 25 Jul 2018
    12. Shuang Wang,Shuaiwei Ren,Raoxian Bai,Puhao Xiao,Qin Zhou,Yin Zhou,Zhigang Zhou,Yuyu Niu,Weizhi Ji and Yongchang Chen.No off-target mutations in functional genome regions of aCRISPR/Cas9-generated monkey model of musculardystrophy. JBC ACCELERATED COMMUNICATION. 293,11654-11658. June 25, 2018
    13. Yiqiang Cui,Yuyu Niu,Jiankui Zhou,Yongchang Chen, Yiwei Cheng, Shangang Li,Zongyong Ai, Chu Chu, Hong Wang, Bo Zheng,Xuejin Chen, Jiahao Sha, Xuejiang Guo,Xingxu Huang, Weizhi Ji. Generation of a precise Oct4-hrGFP knockin cynomolgusmonkey model via CRISPR/Cas9-assisted homologous recombination. Cell Research.28,(2018):383-386.
    14. Xufeng Fu, Bin Jiang, Bingrong Zheng, Yaping Yan, Junfeng Wang,Yanchao Duan, Shanshan Li, Li Yan, Hong Wang, Bingbing Chen,Xiongbo Sang, Weizhi Ji, Ren-He Xu* and Wei Si*. Heterogenic transplantation of bone marrow-derived rhesus macaque mesenchymal stem cells ameliorates liver fibrosis induced by carbon tetrachloride in mouse. Peer J.6:e4336. February 12, 2018
    15. Yu Kang, Zongyong Ai, Kui Duan, Chenyang Si, Yong Wang, Yun Zheng, Jingjing He, Yu Yin, Shumei Zhao, Baohua Niu, Xiaoqing Zhu, Li Liu, Lifeng Xiang, Linming Zhang, Yuyu Niu, Weizhi Ji, and Tianqing Li*. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys. Cell Reports. 25(9),2563-2576,27 November 2018
    16. Yuyu Niu, Tianqing Li and Weizhi Ji*.Paving the road for biomedicine: genome editing and stem cells in primates[J]. Stem Cell Research. Volume 4, Issue 4, July 2017, 543–549, 25 August 2017
    17. Gao F,Niu YY,Sun YE,Lu HL,Chen YC,Li SG,Kang Y, Luo YP,Si CY, Yu JH,Li C,Sun NQ,Si W,Wang H,Ji WZ*,Tan T*.De novo DNA methylation during monkey pre-implantation embryogenesis[J]. Cell Res. 27, 526–539(2017),
    18. Chen YC*,Yu JH,Niu YY,Qin DD,Liu HL,Li G,Hu YZ,Wang JJ,Lu Y,Kang Y, Jiang Y,Wu KH,Li SG,Wei JK,He J,Wang JB,Liu XJ,Luo YP,Si CY,Bai RX,Zhang KS,Liu J,Huang SY,Chen ZZ,Wang S,Chen XY,Bao XH,Zhang QP,Li FX,Geng R,Liang AB,Shen DG,Jiang TZ,Hu XT,Ma YY,Ji WZ*,Sun YE*. Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys[J]. Cell. Volume 169, Issue 5, 945-955.e10, 18 May 2017
    19. Chen B, Li S, Yan Y, Duan Y, Chang S, Wang H, Ji W*, Wu X*, Si W*.Cryopreservation of cynomolgus macaque (Macaca fascicularis) sperm with glycerol and ethylene glycol, and its effect on sperm-specific ion channels - CatSper and Hv1. Theriogenology. Volume 104, 37-42,, December 2017
    20. Zhuo Y,Feng SQ,Huang SY,Chen XL,Kang Y,Si CY,Li ZF,Zhou Y,Zhou L,Zhang T,Ji WZ,Niu YY*,Chen YC*.Transabdominal Ultrasound-guided Multifetal Pregnancy Reduction in 10 Cases of Monkeys. Biology of Reproductio. Volume 97, Issue 5, November 2017, 758–761, 23 October 2017
    21. Fu X, Yan Y, Li S, Wang J, Jiang B, Wang H, Duan Y, Tan T, Gao F, Gong D, Niu Y, Ji W*, Zheng B, Si W*.Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression. Stem Cells International. Volume 2017,14 ,24 October 2017
    22. Chen Y, Niu Y, Ji W. Genome editing in nonhuman primates: approach to generating human disease models[J]. J Intern Med. Volume280, Issue3,September 2016, 246-251. 25 April 2016
    23. Zhu XQ,Li B,Ai ZY,Xiang Z,Zhang KS,Qiu XY,Chen YC,Niu YY,Hu XT, Sun YE,Ji WZ*,Li TQ*.A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies[J]. Stem Cell Reports. Volume 6, Issue 2,228-242, 9 February 2016
    24. He JJ,Xiang Z,Zhu XQ,Ai ZY,Shen JS,Huang TZ,Liu LG,Ji WZ,Li TQ*. Neuroprotective Effects of 7, 8-dihydroxyflavone on Midbrain Dopaminergic Neurons in MPP+-treated Monkeys[J].Scientific Reports.6,34339 (2016),12 October 2016
    25. Ai ZY,Xiang Z,Li YM,Liu GK,Wang H,Zheng Y,Qiu XY,Zhao SM,Zhu XQ,Li YH,Ji WZ,Li TQ*. Conversion of monkey fibroblasts to transplantable telencephalic neuroepithelial stem cells[J]. Biomaterials. Volume 77, January 2016, Pages 53-65.
    26. Lu HL,Yuan ZM,Tan T,Wang JW,Zhang, JY,Luo HJ,Xia YD,Ji WZ,Gao F*. Improved tagmentation-based whole-genome bisulfite sequencing for input DNA from less than 100 mammalian cells[J]. Epigenomics. Vol.7,No.1:47-56.17 Feb 2015
    27. Zhang JJ,Li TJ,Ji WZ,Yu Y*,Tan T*. Rho GDIalpha Modulates Rabbit Trophoblast Stem Cell Survival and Migration[J]. Biol Reprod. Volume 93, Issue 6, 144, 1-9, 01 December 2015
    28. Jiang B,Xiang Z,Ai ZY,Wang H,Li YJ,Ji WZ*,Li TQ. Generation of cardiac spheres from primate pluripotent stem cells in a small molecule-based 3D system[J]. Biomaterials.Volume 65, October 2015, Pages 103-114.
    29. Chen YC,Zheng YH, Kang Y,Yang WL,Niu YY,Guo XY,Tu ZC,Si CY,Wang H,Xing RX,Pu XQ,Yang SH,Li SH,Ji WZ,Li XJ*. Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9[J]. Hum Mol Genet. Volume 24, Issue 13, 1 July 2015, Pages 3764–3774. 09 April 2015
    30. Chen YC,Niu YY,Li YJ,Ai ZY,Kang Y,Shi H,Xiang Z ,Yang ZH,Tan T,Si W,Li W,Xia XS,Zhou Q*,Ji WZ,Li TQ. Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells[J]. Cell Stem Cell. Volume 17, Issue 1, 2 July 2015, Pages 116-124
    31. Niu YY,Guo XY,Chen YC,Wang CE,Gao JQ,Yang WL,Kang Y ,Si W,Wang H,Yang SH,Li SH,Ji WZ*,Li XJ. Early Parkinson's disease symptoms in alpha-synuclein transgenic monkeys[J]. Hum Mol Genet. Volume 24, Issue 8, 15 April 2015, Pages 2308–2317,30 December 2014
    32. Wan HF,Feng CJ,Teng F,Yang SH,Hu BY,Niu YY,Xiang AP,Fang WZ,Ji WZ,Li W,Zhao XY,Zhou Q*. One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system[J]. Cell Res. 25, 258–261 (2015),28 November 2014
    33. Chen YC,Cui YQ,Shen B,Niu YY,Zhao XY,Wang L,Wang JY,Li W,Zhou Q, Ji WZ,Sha JH,Huang XX*. Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys[J]. Cell Res. 25, 262–265 (2015),23 December 2014
    34. Kang Y,Zheng B,Shen B, Chen YC,Wang L,Wang JY,Niu YY,Cui YQ,Zhou JK, Wang H,Guo XJ,Hu B,Zhou Q,Sha JH,Ji WZ,Huang, XX*. CRISPR/Cas9-mediated Dax1 knockout in the monkey recapitulates human AHC-HH[J]. Hum Mol Genet. Volume 24, Issue 25, 20 December 2015, Pages 7255–7264
    35. Wang H,Tan T,Wang JF,Niu YY,Yan YP,Guo XY,Kang Y,Duan YC,Chang SH,Liao JP,Si CY,Ji WZ*,Si W. Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis[J]. Sci Rep. 5, 15019 (2015),07 October 2015
    36. Liu HL,Chen YC,Niu YY,Zhang KS,Kang Y,Ge WH,Liu XJ,Zhao EF,Wang CC,Lin SY,Jing B,Si CY,Lin Q,Chen XY,Lin HJ,Pu XQ,Wang YY,Qin BL,Wang F,Wang H,Si W,Zhou J,Tan T,Li TQ,Ji SH,Xue ZG,Luo YP,Cheng LM,Zhou Q,Li SG*,Sun YE,Ji WZ. TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys[J]. Cell Stem Cell. Volume 14, Issue 3, 6 March 2014, Pages 323-328
    37. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X*, Ji W*, Sha J*. Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Cell, Volume 156, Issue 4, 836-843, 30 January 2014
    38. Liu H, Chen Y, Niu Y, Zhang K, Kang Y, Ge W, Liu X, Zhao E, Wang C, Lin S, Jing B, Si C, Lin Q, Chen X, Lin H, Pu X, Wang Y, Qin B, Wang F, Wang H, Si W, Zhou J, Tan T, Li T, Ji S, Xue Z, Luo Y, Cheng L, Zhou Q, Li S*, Sun YE*, Ji W*. TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys. Cell Stem Cell, Volume 14, Issue 3, 323-328, 13 February 2014
    39. Zhang YF,Zhang Y,Shi JC,Zhang H, Cao ZH,Gao X, Ren WH,Ning YN,Ning LN,Cao YJ,Chen YC,Ji WZ,Chen ZJ,Chen Q,Duan, EK*. Identification and characterization of an ancient class of small RNAs enriched in serum associating with active infection[J]. J Mol Cell Biol. Volume 6, Issue 2, April 2014, Pages 172–174,30 December 2013
    40. Tan T,Lu B,Zhang J,Niu YY,Si W,Wei Q,Ji WZ*. Notch1 signaling antagonizes transforming growth factor-beta pathway and induces apoptosis in rabbit trophoblast stem cells[J]. Stem Cells Dev. Volume: 23 Issue 8: November 19, 2013
    41. Tan T, Lu B, Zhang J, Niu YY, Si W, Wei Q, Ji WZ 2013 Notch1 Signaling Antagonizes Transforming Growth Factor-β Pathway and Induces Apoptosis in Rabbit Trophoblast Stem Cells. Stem Cells and Development.doi:1089/scd.2013.0280
    42. Chen Y, Niu Y, Ji W. 2012 Transgenic nonhuman primate models for human diseases: approaches and contributing factors. J Genet Genomics. 39(6):247-51.
    43. Chen Y, Niu Y, Yang S, He X, Ji S, Si W, Tang X, Xie Y, Wang H, Lv Y, Zhou Q and Ji W. 2012 The Available Time Window for Embryo Transfer in the Rhesus Monkey (Macaca mulatta). American Journal of Primatology. 74 (2):165–173
    44. Chen Y, Wang Z, Xie Y, Guo X, Tang X, Wang S, Yang S, Chen K, Niu Y, Ji W.2012Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells. J Neurosci Res. 90(7):1382-91
    45. Tn T, Tang X, Zhang J, Niu Y, Chen H, Li B, Wei Q, Ji W. 2011 Generation of trophoblast stem cells from rabbit embryonic stem cells with BMP4. Plos One Volume 6 | Issue 2 | e17124.
    46. Yang S, Ping S, Si W, He X, Wang X, Lu Y, Ji S, Niu Y, Ji W. 2011 Optimization of ethylene glycol concentrations, freezing rates and holding times in liquid nitrogen vapor for cryopreservation of rhesus macaque (Macaca mulatta) sperm. J Vet Med Sci. 73(6):717-23.
    47. Yang S, Ping S, Ji S, Lu Y, Niu Y, Wang H, Ji W, Si W. 2011 The positive effects of seminal plasma during the freezing process on cryosurvival of sperm with poor freezability in the rhesus macaque (Macaca mulatta). J Reprod Dev. 57(6):737-43.
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