孙正龙(Zhenglong Sun)

孙正龙，博士，教授，云南省青年兴滇英才计划。研究方向：显微病理图像处理，前沿光学显微成像技术及其在细胞生物学、发育生物学中的应用，黏液型肿瘤。近五年来，作为核心项目骨干成员，参与国家重大科研装备研制项目“超分辨显微光学核心部件及系统研制”，个人负责项目中的样机性能调试，生物学检测，生物医学应用等工作。具体参与DMD-SIM 超分辨显微镜、非线性SIM 超分辨显微镜、激光干涉SIM 超分辨显微镜、STED 超分辨显微镜、STORM 超分辨显微镜、双光子-光片显微镜等大型设备研制。个人主持国家自然科学基金青年项目，中科院仪器功能开发项目，昆明理工大学人才特区项目，企业横向课题等项目8项。

• 现任昆明理工大学非人灵长类生物医学国家重点实验室，教授；
• 2015-2020中国科学院苏州生物医学工程与技术研究所江苏省医用光学重点实验室副研究员，硕士生导师；    
• 2012-2015清华大学生物力学与医学工程研究所博士后；   

1） 昆明理工大学人才启动经费。主持

2） 中科院上海营养与健康研究所来源横向项目，适于高内涵成像系统的自动移液装置开发，2020/03-2020/08, 18.5万元，结题。主持

3） 中国科学院苏州生物医学工程技术研究所所立基础科研项目，722141202，异甘草素治疗骨肉瘤效果评价，2018/06-2020/05，20万元, 结题。主持

4） 中国科学院仪器功能开发专项，752091205，基于激光共聚焦显微镜的活细胞力学工作站，2017/09-2018/08，60万元, 已结题。主持

5） 国家自然科学基金青年项目，31400799，EHD2在细胞外基质弹性诱导干细胞分化过程中作用，2015/01-2017/12, 23万元，结题。主持

6） 中科院昆明植物所来源横向项目，化学结晶仪可视化成像设备研制，2019/01-2019/12, 18万元，结题。主持

7） 苏州医工所-苏州高新区人民医院医工结合项目，EHD2调控MSCs迁移的机制研究，2019/08-2020/07, 10万元，在研。主持

8） 科技部国家重大科研仪器研制项目：超分辨显微镜及其核心部件（2014年1月-2018年12月）。项目金额：1亿7280万元。参与。

1)Zhenyu Yang，Miao Guan，Zhenglong Sun*. Research Progress of Expansion Microscopy(2022). Progress of Biochemistry and biophysics(accept)

2)H. Yang, X. Peng, A.J. Gooday, B. Jones，J. Li, S. Liu ,W. Huang, Z. Sun, S. Chen, S. Dasgupta, H. Xu, S. Liu, W. Xu, K. Ta1Yang H, Peng X, Gooday A J, et al. Magnetic foraminifera thrive the Mariana Trench. GEOCHEMICAL PERSPECTIVES LETTERS, 2022, 21: 23-27.

3)Xiao Luo，Yan Chen，Yanchun Li，Zhenglong Sun*，Weihong Zhu，Xuhong Qian*，Youjun Yang* (2020). Structurally-thrifty and visible-absorbing fluorophores.Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,Volume 245, 15 January 2021, 11890

4)YanshengXiao, YanchunLi, FangHu, XiaoLuo*,ZhenglongSun*, Xu HongQian, YoujunYang*.A Nucleolus-Targeting Hill-type pH Probe.Sensors and Actuators B:Chemical.Volume 335, 15 May2021, 129712

5) Yiran Gu，Long Zhang，Hua Yang，Jie Zhuang，Zhenglong Sun*，Jinsong Guo*，Miao Guan*. Nanosecond pulsed electric fields impair viability and mucin expression in mucinous colorectal carcinoma cell. Bioelectrochemistry.Volume 141, October 2021, 107844

6) Gang Wen, Simin Li, Linbo Wang, XiaohuChen,Zhenglong Sun, Yong Liang, Xin Jin, YuguoTang, Hui Li.High-fidelity structured illumination microscopy by point-spread-function engineering[J]. Light: Science & Applications, 2021, 10(1): 1-12.

7) Yong Liang, Xiaohu Chen,Zhenglong Sun, Chong Chen, Linbo Wang, Simin Li.Liang Y, Chen X, Sun Z, et al. High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures[J]. Frontiers in Physics, 2021, 9: 648174.

8) Zhitao Li,GuoaoHu, LiZhu,Zhenglong Sun, YunJiang, MinjieGao, Xiaobei Zhan. Study of growth, metabolism, and morphology of Akkermansia muciniphila with an in vitro advanced bionic intestinal reactor[J]. BMC microbiology, 2021, 21(1): 1-12.

9) Zhu C, Wang A, Chen L, Guo L, Ye J, Chen Q, Wang Q, Yao G, Xia Q, Cai T, Guo J, Yang Z,Sun Z, Xu Y, Lu G, Zhang Z, Cao J, Liu Y, Xu H. Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks. J Biophotonics. 2021 Apr 15:e202100001.

10) Yansheng Xiao，FangHu,Xiao Luo, MingmingZhao,ZhenglongSun*, XuhongQian*, YoujunYang*. Modulating the pKa’s of Hill-type pH probes for Bio-relevant Acidic pH Range.ACS Applied Bio Materials.(2020,https://doi.org/10.1021/acsabm.0c01262)

11) Yiwei Chen,Zhenglong Sun, Yi He, Xin Zhang, Jing Wang, Wanyue Li, Lina Xing, Feng Gao, Guohua Shi.Automated Nuclear Lamina Network Recognition and Quantitative Analysis in Structured Illumination Super-Resolution Microscope Images Using a Gaussian Mixture Model and Morphological Processing.Photonics 2020, 7(4), 119

12) Peng X, Yan X, Zhu L, Gu Y,Sun Z, Zhan X. Preparation of polymyxin B-loaded gellan-polylysine polyion complex fibers with high affinity to endotoxin. Int J Biol Macromol. 2020 Oct 1;160:703-710.

13) Peng X, Zhu L, Guo J,Sun Z, Zhao M, Zhan X. Enhancing biocompatibility and neuronal anti-inflammatory activity of polymyxin B through conjugation with gellan gum. Int J Biol Macromol. 2020 Mar 15;147:734-740.

14) Lei Y, Wu M, Wang J, Zhang H, Zhan X,Sun Z, Wu J. Preparation and property of a biantenna macromolecule based on polysialic acid. Int J Biol Macromol. 2020 Jul 15;155:1342-1349.

15)顾依然，杨华，陆维祺，张伟，史国华*，孙正龙*(2020)。腹膜假粘液瘤基础研究进展。肿瘤学杂志.2020,26(10):907-913

16) Guo, Q., Zhu, Q., Miao, T., Tao, J., Ju, X.,Sun, Z., ... & Han, L. (2019). LRP1-upregulated nanoparticles for efficiently conquering the blood-brain barrier and targetedly suppressing multifocal and infiltrative brain metastases. Journal of Controlled Release, 303, 117-129.

17) Yang, X., Zhu, Q.,Sun, Z., Wen, G., Jin, X., Wang, L., ... & Li, H. (2019). Fringe optimization for structured illumination super-resolution microscope with digital micromirror device. Journal of Innovative Optical Health Sciences, 12(03), 1950014.

18) Qiu, S. B.; Dai, D. D.; Guo, C. L.;Sun, Z. L.; Li, H.; Qian, X. H.; Yang, Y. J., ( 2019) Amino-substituted C-coumarins: Synthesis, spectral characterizations and their applications in cell imaging. Dyes Pigments, 163, 55-61.

19) Wang, L.; Li, S.; Sun, Z.; Wen, G.; Zheng, F.; Fu, C.; Li, H., (2018) Segmentation of yeast cell's bright-field image with an edge-tracing algorithm. Journal of biomedical optics, 23 (11), 1-7.

20) Qiu, S. B.; Guo, C. L.; Wang, M. K.;Sun, Z. L.; Li, H.; Qian, X. H.; Yang, Y. J., (2018) Mild dealkylative N-nitrosation of N, N-dialkylaniline derivatives for convenient preparation of photo-triggered and photo-calibrated NO donors. Org Chem Front, 5 (22).

21)Guo, Q.; Chang, Z.; Khan, N. U.; Miao, T.; Ju, X.; Feng, H.; Zhang, L.;Sun, Z.; Li, H.; Han, L.,( 2018) Nanosizing Noncrystalline and Porous Silica Material-Naturally Occurring Opal Shale for Systemic Tumor Targeting Drug Delivery. ACS applied materials & interfaces, 10 (31), 25994-26004.

22)孙正龙#，李思黾，李辉（2018）。DMD-结构光照明超分辨显微成像。第十一届中国生命科学公共平台管理与发展研讨会会议论文。

23) Jiang, L.#, Sun, Z.L.#,Chen, X.F.,Li, J., Xu, Y., Zu, Y., Hu, J.L.,Han, D. *& Yang, C*. (2016)Cells Sensing Mechanical Cues: Stiffness Influences the Lifetime of Cell-Extracellular Matrix Interactions by Affecting the Loading Rate. ACS NANO.10 (1): 207-17（Co-first author）

生物检测技术与设备，前沿显微成像技术