艾宗勇 (Zongyong Ai)

• 2005年9月-2009年7月 大连理工大学环境与生命学院，生物技术，学士
• 2009年8月-2011年3月 浙江海正药业股份有限公司，技术员
• 2011年3月-2016年9月 云南中科灵长类生物医学重点实验室，助理研究员
• 2016年9月-2021年6月 昆明理工大学，生物学，博士
• 2021年7月-至今 昆明理工大学灵长类转化医学研究院，副教授

• 09/2005-07/2009 B.S., School of Environment and Life, Dalian University of Technology
• 08/2009-03/2011 Technician, Zhejiang Hisun Pharmaceutical Co., Ltd.
• 03/2011-09/2016 Assistant professor, Yunnan Key Laboratory of Primate Biomedical Research
• 09/2016-06/2021 Ph.D., Institute of Primate Translational Medicine, Kunming University of Science and Technology
• 07/2021-present Associate Professor, Institute of Primate Translational Medicine, Kunming University of Science and Technology

1. 灵长类干细胞（多能干细胞和胚外干细胞）
2. 灵长类早期胚胎发育
3. 灵长类的类胚胎

1. Primate stem cells (Pluripotent stem cells and Extraembryonic stem cells)
2. Primate early embryogenesis
3. Primate stem cell–based embryo models

人早期胚胎发育异常是不育、流产和出生缺陷的主要原因。然而，我们对人早期胚胎发育过程知之甚少。

囊胚中的滋养外胚层、下胚层和上胚层具有卓越的发育潜能，三者能够协同发育产生胎盘、卵黄囊和胎儿。因此，它们对应的体外培养物——滋养层干细胞、原始内胚层干细胞和多能干细胞在早期胚胎发育研究和再生医学领域拥有广阔前景：基于它们的发育潜能，可以通过三种干细胞深入探索早期胚胎的关键发育事件并揭示胚胎异常发育的机制；基于它们强大的增殖和分化能力，可以通过三种干细胞制备大量的功能细胞或细胞产品，用于再生医学。

因此，我们聚焦灵长类早期胚胎发育和三种类型干细胞的研究，以期践行生命科学研究的两大使命：探索人类生命发生的奥秘和造福人类健康。

Abnormal early embryonic development in humans is a major cause of implantation failure, early pregnancy loss, miscarriage and birth defects. However, we know very little about the process of early human development.

The trophectoderm, hypoblast and epiblast in the blastocyst have excellent developmental potential, and the three can synergistically develop into the placenta, yolk sac and fetus, respectively. Accordingly, their in vitro counterparts—trophoblast stem cells, primitive endoderm stem cells and pluripotent stem cells, hold great promise in the fields of early embryonic development research and regenerative medicine: (1) based on their developmental potential, these three types of stem cells can be used to deeply explore the key events during early embryo development and reveal the mechanisms of abnormal development; and (2) based on their great proliferation and differentiation capacity, these three kinds of stem cells can produce a large number of various functional cells or cell products in vitro for regenerative medicine.

Therefore, we focus on the research on primate early development and three types of stem cells, in order to fulfill the two major missions of life science research: exploring the mystery of life and benefiting human health.

四、承担科研项目情况 (ResearchFunding)

1. 国家自然科学基金委员会，地区科学基金项目，31760268，猴体细胞无外源基因导入条件下转分化为功能神经上皮干细胞的研究，2018-01-01至2021-12-31，36万元，结题，主持

2. 国家自然科学基金，重大项目，82192874，胚胎植入后发育与不良妊娠结局，2022-01-01至2026-12-31，450万元，在研，研究骨干

3. 国家重点研发计划，基于疾病进程的重大神经性致盲眼病干细胞治疗临床前研究，2020YFA0112700，2020-10 至 2024-12，1826万元，在研，研究骨干

4. 国家重点研发计划，类胚胎标准化制备及其在疾病机理研究和药物研发中的应用，2022YFA1103100，2022-10 至 2027-10，2791.50万元，在研，研究骨干

5. 国家自然科学基金，重点项目，32130034，利用3D延迟培养和“人工”胚胎揭示人着床胚胎发育和调控机制，2022-01-01至2026-12-31，287万元，在研，参与

1. 李天晴，季维智，牛昱宇，陈永昌，艾宗勇，朱小庆，康宇。灵长类干细胞的多能性、分化和再生机制，云南省自然科学奖，一等奖，2021。

1. Ai Z^#, Xiang Z^#, Li Y^#, Liu G, Wang H, Zheng Y, Qiu X, Zhao S, Zhu X, LiY, Ji W*, Li T*.Conversion of monkey fibroblasts to transplantable telencephalic neuroepithelial stem cells[J]. Biomaterials, 2016, 77: 53-65. IF=8.402，中科院JCR一区，第一作者。
2. Ai Z^#, Niu B^#, Duan K^#, Si C^#, Wang S, Xiang L, Zhu X, Zhu Q, Feng C, Yin Y,Zhao S, Kong R, Ji W*, Li T*.Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells[J]. Biomaterials, 2020, 249(120015). IF=10.317，中科院JCR一区，第一作者。
3. Ai Z; Yin Y; Niu B; Li T*. Deconstructing humanperi-implantation embryogenesis based on embryos and embryoids[J]. Biology ofReproduction, 2022, 107(1): 212-225. IF=4.161，中科院JCR二区，第一作者。
4. Zhu X^#, Li B^#, Ai Z^#, Xiang Z, Zhang K, Qiu X, Chen Y, Li Y, Rizak JD, Niu Y, Hu X, Sun YE, JiW*, Li T*. ARobust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies[J]. StemCell Reports, 2016, 6 (2):228-242. IF=7.338，中科院JC一区，共同一作。
5. Kang Y^#, Ai Z^#, Duan K^#, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, NiuB, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W*, Li T*. Improving Cell Survival in Injected EmbryosAllows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys[J]. Cell Rep, 2018, 25 (9): 2563-2576 e2569. IF=7.815，中科院JCR一区，共同一作。
6. Chen Y^#, Niu Y^#, Li Y^#, Ai Z, Kang Y, Shi H, Xiang Z, Yang Z,Tan T, Si W, Li, W, Xia X, Zhou Q*, Ji W*, Li T*. Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells[J]. Cell Stem Cell, 2015,17, 116-24.
7. He J, Xiang Z, Zhu X, Ai Z, Shen J, Huang T, Liu L, Ji W*, Li T*. Neuroprotective Effectsof 7, 8-dihydroxyflavone on Midbrain Dopaminergic Neurons in MPP+-treated Monkeys[J]. Sci Rep, 2016, 6: 34339.
8. Zhu X, Ai Z,Hu X*, Li T*. Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells[J]. Sci Rep,2016, 6: 28572.
9. Cui Y^#, Niu Y^#, Zhou J^#,Chen Y^#, Cheng Y, Li S, Ai Z,Chu C, Wang H, Zheng B, Chen X, Sha J, Guo X*, Huang X*, Ji W*. Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination[J]. Cell Res,2018, 28 (3): 383-386.
10. Li T*, Ai Z,Ji W*. Primate stem cells: bridge the translation from basic research to clinic application[J]. Sci China Life Sci, 2019, 62 (1): 12-21. 
11. Xiang L^#, Yin Y^#, Zheng Y^#,Ma Y^#, Li Y^#, Zhao Z, Guo J, Ai Z, Niu Y, Duan K, He J, Ren S, Wu D, Bai Y, Shang Z, Dai X, Ji W*,Li T*. A developmental landscape of 3D-cultured human pre-gastrulation embryos[J]. Nature, 2020, 577 (7791): 537-542.
12. Zhao S^#, Duan K^#, Ai Z, Niu B, Chen Y, Kong R, Li T*.Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells[J]. Stem Cell Res Ther, 2020,11 (1): 431.
13. Tan T^#*, Wu J^#*, Si C^#,Dai S^#, Zhang Y^#, Sun N, Zhang E, Shao H, Si W, Yang P,Wang H, Chen Z, Zhu R, Kang Y, Hernandez-Benitez R, Martinez L, Delicado E, Berggren W, Schwarz M, Ai Z, Li T,Esteban C, Ji W*, Niu Y*, Izpisua Belmonte J. C*. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo[J]. Cell, 2021, 184, 2020-2032 e2014.

1. 李天晴；艾宗勇；牛宝华；多能干细胞的培养基，培养方法及其应用，2021.3.19-2039.8.21，中国，CN201910772540.4