司晨洋

2024年01月-至今，昆明理工大学灵长类转化医学研究院，副教授

2017年09月-2023年06月，昆明理工大学，生物学，博士

2010年08月至2017年08月，云南中科灵长类生物医学重点实验室，助理研究员

2009年07月-2010年07月，成都天邦生物制品有限公司，技术员

2005年09月-2009年06月，吉首大学，生物工程，学士

01/2024-Present, Associate Professor, Institute of Primate Translational Medicine, Kunming University of Science and Technology

09/2017-06/2023, Ph.D., Institute of Primate Translational Medicine, Kunming University of Science and Technology

08/2010-08/2017, Assistant professor, Yunnan Key Laboratory of Primate Biomedical Research

07/2009-07/2010, Technician, Chengdu Tianbang Biological Products Co., Ltd.

09/2005-06/2009, Bioengineering, Jishou University.

灵长类分割胚胎发育潜能及其调控机制研究

Research on the developmental potential and regulatorymechanisms of primate split Embryos

植入前胚胎在一定程度干扰后仍然具备发育为完整个体的潜能，胚胎分割技术利用这种特性，将单个胚胎分割为两份或多份以产生同卵双胎或多胎。已在兽医领域成熟应用，但在灵长类（包括人类和非人灵长类）中仍面临伦理和技术瓶颈。我们综合运用单细胞测序、干细胞嵌合和基因编辑等技术，系统解析猴同源分割胚胎的发育潜能异质性，解析关键调控机制并开发优化方案。旨在从单个猴胚胎获得两个个体（即同卵双胞胎），提高胚胎利用率。为解决辅助生殖领域中移植胚胎资源短缺问题提供新路径；同步获得遗传背景匹配的疾病模型个体与野生型对照，消除遗传变异干扰，推动复杂疾病早期诊断标志物的发现。

Preimplantation embryos retain the ability to develop into complete individuals even after manipulation. Embryo splitting takes advantage of this property by dividing a single embryo into two or more parts to produce monozygotic multiples. Although this method is widely used in veterinary medicine, its use in primates—including humans and non-human primates—is still constrained by ethical and technical issues. By combining single-cell sequencing, stem cell chimeras, and gene editing, we aim to explore the differences in developmental potential among homologous monkey split embryos, understand the key regulatory mechanisms, and develop improved strategies. Our objective is to generate two individuals from one monkey embryo, thereby increasing embryo utilization efficiency. This strategy may help address the shortage of embryos for transfer in assisted reproduction and allows for the simultaneous production of genetically matched disease model individuals and wild-type controls, reducing genetic background variation and aiding in the identification of early diagnostic markers for complex diseases.

国家自然科学基金委员会, 地区科学基金项目, 32460179, 基于胚胎分割技术建立非人灵长类自体胚胎干细胞的研究, 2025-01-01 至 2028-12-31, 32万元, 在研, 主持

1. Si, C., Zhu, R., Wu, J. et al. Production of live monkeys and their genetically matched embryonic stem cells from single embryos. Nature Communications, 2025. 16, 5529. IF=15.7，中科院JCR一区。

2. Tan T.#*, Wu J.#*, Si C.*, Dai S.*, Zhang Y.*, Sun N. et al., Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo. Cell, 2021. 184(13): 3589. IF=66.85，中科院JCR一区。

3. Ai Z.*, Niu B.*, Duan K.*, Si C.*, Wang S., Xiang L. et al., Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells. Biomaterials, 2020. 249: 120015. IF=10.317，中科院JCR一区。

4. Zhu X.*, Guo Y.*, Chu C.*, Liu D.*, Duan K., Yin Y., Si C., Kang Y. et al., BRN2 as a key gene drives the early primate telencephalon development. Science Advances, 2022. 8(9): eabl7263.

5. Yang R.*, Goedel A.*, Kang Y.*, Si C., Chu C. et al., Amnion signals are essential for mesoderm formation in primates. Nature Communications, 2021. 12(1): 5126.

6. Chu C.*, Zhang W.#*, Kang Y., Si C., Ji W.#, Niu Y.#, and Zhang Y.#, Analysis of developmental imprinting dynamics in primates using SNP-free methods to identify imprinting defects in cloned placenta. Developmental Cell, 2021. 56(20): 2826-2840 e2827.

7. Niu Y.#*, Sun N.*, Li C.*, Lei Y.*, Huang Z., Wu J., Si C., Dai X. et al., Dissecting primate early post-implantation development using long-term in vitro embryo culture. Science, 2019. 366(6467).

2017年云南省自然科学奖一等奖；非人灵长类基因编辑与神经系统疾病模型研究，排名第七。

牛昱宇；司晨洋；吴俊模；基于胚胎分割技术建立非人灵长类自体胚胎干细胞的方法，2024.09.06-2044.09.06，中国，CN202410668256.3